PMID: 2511558Nov 25, 1989Paper

Characterization of amplified intracisternal A-particle elements encoding integrase

Nucleic Acids Research
K K LuedersJ W Fewell

Abstract

Type IIB intracisternal A-particle (IAP) elements have undergone marked amplification and transposition in the genomic DNA of some mouse myelomas. We have made a cDNA library from one such myeloma, MOPC 315, to determine whether some property of the elements themselves has a role in this process. Sequencing of several type IIB cDNAs and one genomic type IIB IAP element has shown that they are nearly identical (greater than 99%) and contain 2 open reading frames (ORFs). ORF2 is capable of encoding the IAP integrase, an enzyme which catalyzes integration of proviral DNA into the genome. An antiserum to a synthetic peptide based on the IAP integrase gene sequence reacted with ORF2 product expressed in bacteria as a fusion protein, and detected a 47 kDa protein, predicted from the size of ORF2, in myeloma cell fractions by Western blotting.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N TaneseS P Goff
Apr 1, 1989·Genes & Development·B BowermanH E Varmus
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E L KuffC L Martens
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki

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Citations

Feb 1, 1991·Developmental Biology·A A Poznanski, P G Calarco
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·I HakimG Rechavi
Mar 5, 2008·Shock·Kiho ChoDavid G Greenhalgh

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