PMID: 2492438Jan 27, 1989Paper

Characterization of an 8-hydroxy-5-deazaflavin:NADPH oxidoreductase from Streptomyces griseus

Biochimica Et Biophysica Acta
A P EkerR Meerwaldt

Abstract

An 8-hydroxy-5-deazaflavin-dependent oxidoreductase was isolated from the actinomycete Streptomyces griseus and purified 590-fold with 72% overall yield. The enzyme catalyzes electron transfer between 8-hydroxy-5-deazaflavins and NADPH. It seems to be more specific than methanogenic oxidoreductase as it has an absolute requirement for both the 5-deazaflavin structure and the presence of an 8-hydroxy group in the substrate. A molecular weight of 42,000 was found with gel permeation chromatography, while SDS gel electrophoresis indicated the presence of two identical subunits. Maximal enzymatic activity was found at 0.32 M NaCl and pH 5.9 for reduction of 8-hydroxy-5-deazaflavin and pH 7.9 for the reverse reaction. From the kinetic constants it was estimated that the main function of this oxidoreductase is probably to provide cells with reduced 8-hydroxy-5-deazaflavin to be used in specific reduction reactions. These results indicate the occurrence of 8-hydroxy-5-deazaflavin-dependent electron transfer in microorganisms not belonging to the archaebacteria.

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Citations

Oct 20, 2005·Journal of Bioscience and Bioengineering·Seigo ShimaUlrich Ermler
Oct 31, 2002·Applied and Environmental Microbiology·Dale IsabelleLacy Daniels
May 7, 2003·Applied and Environmental Microbiology·Gesche HeissHans-Joachim Knackmuss
Dec 8, 2000·Canadian Journal of Microbiology·D A EliasR Sparling
Jun 25, 2004·Bioscience, Biotechnology, and Biochemistry·Tetsuo NakanoRyoichi Katsumata
Jun 13, 2008·DNA Repair·Monique M van OersAndré P M Eker
Sep 1, 1992·Analytical Biochemistry·E PurwantiniL Daniels
Jul 13, 2005·Natural Product Reports·Markus Fischer, Adelbert Bacher
Apr 29, 2016·Microbiology and Molecular Biology Reviews : MMBR·Chris GreeningColin J Jackson
Apr 16, 2017·The Journal of Biological Chemistry·Hemant KumarMarco W Fraaije
Apr 15, 2021·FEMS Microbiology Reviews·Rhys Grinter, Chris Greening

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