PMID: 9434948Jan 22, 1998Paper

Characterization of an abundant short interspersed nuclear element (SINE) present in Canis familiaris

Mammalian Genome : Official Journal of the International Mammalian Genome Society
M DasJ Pelletier

Abstract

A short interspersed nuclear element (Can SINE) of approximately 130-150 bp was cloned and characterized from Canis familiaris. We demonstrate that this element is interspersed, present approximately every 5-8.3 kbp, and many are sufficiently close to allow IRS (interspersed repetitive DNA sequences) PCR. Sequence analysis of > 20 Can SINEs from the dog has identified a conserved region that was used to design oligonucleotides for IRS PCR. Since Can SINEs are not present in human or rodent genomes, IRS PCR using oligonucleotides directed to the conserved region of Can SINEs can be used to simplify analysis of canid DNA in somatic cell hybrids, as well as in large insert cloning vectors. We demonstrate that the canid IRS products are polymorphic and could be developed as genetic markers for filter-based genotyping in this organism.

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Citations

Oct 26, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Li Yu, Ya-Ping Zhang
Sep 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jay N LozierRichard A Morgan
Apr 26, 2000·Molecular Biology and Evolution·J P SlatteryS J O'Brien
Apr 26, 2019·Scientific Reports·K L Bowlt BlacklockM Starkey
Nov 14, 2000·Physiological Genomics·M DasJ Pelletier

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