Characterization of an acetoin reductase/2,3-butanediol dehydrogenase from Clostridium ljungdahlii DSM 13528

Enzyme and Microbial Technology
Yang TanFuli Li

Abstract

Acetoin reductase catalyzes the formation of 2,3-butanediol from acetoin. In Clostridium ljungdahlii DSM 13528, the gene CLJU_c23220 encoding the putative Zn(2+)-dependent alcohol dehydrogenase was cloned and expressed in Escherichia coli. The recombinant enzyme, CLAR, can catalyze the conversion of acetoin to 2,3-butanediol with NADPH as the cofactor. Furthermore, the gene CLJU_c23220 was introduced into Clostridium acetobutylicum ATCC 824 and the transformant was conferred the capacity of 2,3-butanediol production. In batch fermentation the transformant produced up to 3.1g/L of 2,3-butanediol, as well as acetone, butanol and ethanol (ABE, 17.8 g/L) in amounts similar to those produced by the wild type strain. This study provides conclusive evidence at the protein level that CLJU_c23220 is the key gene responsible for the conversion of acetoin to 2,3-butanediol in C. ljungdahlii DSM 13528. Moreover, the C. acetobutylicum ATCC 824 was modified via one-step metabolic engineering to produce 2,3-butanediol without influencing the ABE production.

References

Sep 10, 2003·Structure·Thomas A PaulyVirginia L Rath
Jul 31, 2007·Journal of Microbiological Methods·John T HeapNigel P Minton
Oct 13, 2007·Proteins·Pascal BenkertDietmar Schomburg
Feb 28, 2009·Nature Protocols·Lawrence A Kelley, Michael J E Sternberg
May 12, 2009·Nucleic Acids Research·Pascal BenkertTorsten Schwede
Jan 29, 2011·Biotechnology Advances·Xiao-Jun JiPing-Kai Ouyang
Feb 22, 2011·Applied and Environmental Microbiology·Marco A J SiemerinkServé W M Kengen
Jun 21, 2011·Applied and Environmental Microbiology·Michael KöpkeSéan D Simpson
Oct 11, 2011·Applied Microbiology and Biotechnology·Ben RaoYaling Shen
Mar 28, 2012·Journal of Microbiological Methods·Gu-zhen CuiQiu Cui
Jun 20, 2012·FEBS Letters·Bettina Schiel-Bengelsdorf, Peter Dürre
Sep 20, 2012·New Biotechnology·Sung-Hyuk HanYong-Cheol Park

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Citations

Nov 15, 2011·Current Opinion in Biotechnology·Bryan P TracyEleftherios T Papoutsakis
Mar 8, 2019·PLoS Computational Biology·Joanne K LiuKarsten Zengler
Apr 19, 2019·3 Biotech·Gyu Bi LeeHyun Sook Lee
Mar 20, 2019·Applied Microbiology and Biotechnology·Gyu Bi LeeJung-Hyun Lee
Nov 17, 2020·Frontiers in Bioengineering and Biotechnology·Jonathan LoPin-Ching Maness

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