Characterization of an alternatively spliced AATYK mRNA: expression pattern of AATYK in the brain and neuronal cells

Oncogene
S BakerE P Reddy

Abstract

The AATYK gene encodes a tyrosine kinase whose expression is up-regulated during the apoptosis and differentiation of 32Dcl3 myeloblastic cells. Because high levels of AATYK mRNA have also been detected in the brain, and because these transcripts differ in size from that observed in the 32Dcl3 cell line, it was of interest to determine whether this gene encodes mRNAs that are alternatively spliced and whether these mRNAs are expressed in a tissue-specific manner. We have isolated a novel, alternatively spliced AATYK mRNA using cDNA library screening and RT-PCR, whose expression is readily detected in the brain but not myeloid cells. Western blot analysis revealed that the AATYK protein was expressed in virtually all regions of the adult rat brain in which neurons are present, including olfactory bulb, forebrain, cortex, midbrain, cerebellum and pons. Immunohistochemical labeling of adult brain sections showed the highest levels of AATYK expression in the cerebellum and olfactory bulb. Expression of AATYK was also up-regulated as a function of RA-induced neuronal differentiation of p19 embryonal carcinoma cells, supporting a role for this protein in mature neurons and neuronal differentiation.

References

Jan 1, 1987·Annual Review of Neuroscience·M Halpern
Aug 1, 1982·The Journal of Cell Biology·E M Jones-VilleneuveV I Kalnins
Jun 6, 2000·Brain Research. Molecular Brain Research·M RaghunathC Damodar Reddy

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Citations

Apr 3, 2003·Brain Research. Molecular Brain Research·Mineko TomomuraRyoji Yano
Feb 4, 2012·PloS One·Minja ZorcTanja Kunej
Mar 5, 2014·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shuang Ma, Brian P Rubin
Mar 29, 2014·Molecular Biology of the Cell·Tetsuya TakanoShin-Ichi Hisanaga
Nov 27, 2009·Neurochemical Research·Jane P HughesStephen D Skaper
Aug 12, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koji TsutsumiShin-ichi Hisanaga
Mar 10, 2015·Molecular Endocrinology·Cécile JacovettiRomano Regazzi
Oct 18, 2012·Physiological Reviews·Kenneth B Gagnon, Eric Delpire
Oct 24, 2002·The Journal of Biological Chemistry·Hong Wang, David L Brautigan
Jul 28, 2020·Frontiers in Molecular Neuroscience·Shin-Ichi HisanagaMineko Tomomura
Sep 24, 2020·Scientific Reports·Miyuki TakahashiShin-Ichi Hisanaga
Jan 1, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Franz WendlerGeorgios Giamas
Sep 16, 2021·Journal of Biochemistry·Keisuke KomakiShin-Ichi Hisanaga

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