Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Valentina D'AtriDavy Guillarme

Abstract

Brentuximab vedotin (Adcetris) is a cysteine-linked antibody-drug conjugate (ADC) used in the treatment of Hodgkin lymphoma (HL) and systemic anaplastic large cell lymphoma (ALCL). In this study, the drug payload and glycan modifications of this ADC were simultaneously characterized using a unique LC-MS middle-up analysis, involving hydrophilic interaction chromatography (HILIC). This work demonstrates that HILIC is an effective and complementary analytical technique to reversed phase liquid chromatography (RPLC) for subunit-level characterization of immuno-conjugates.

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Citations

Jul 10, 2019·Glycobiology·Noortje de HaanManfred Wuhrer
Jan 9, 2019·Analytical and Bioanalytical Chemistry·Bastiaan L DuivelshofValentina D'Atri
Jun 26, 2021·Pharmacology & Therapeutics·Yiming JinAndrew J Wiemer
Nov 9, 2018·Analytical Chemistry·Valentina D'AtriDavy Guillarme
Sep 3, 2021·Journal of Separation Science·Yutaka Matsuda

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