Characterization of an autonomously activated plant ADP-glucose pyrophosphorylase.

Plant Physiology
Susan BoehleinL Curtis Hannah

Abstract

ADP-glucose pyrophosphorylase (AGPase) catalyzes the rate-limiting step in starch biosynthesis in plants and changes in its catalytic and/or allosteric properties can lead to increased starch production. Recently, a maize (Zea mays)/potato (Solanum tuberosum) small subunit mosaic, MP [Mos(1-198)], containing the first 198 amino acids of the small subunit of the maize endosperm enzyme and the last 277 amino acids from the potato tuber enzyme, was expressed with the maize endosperm large subunit and was reported to have favorable kinetic and allosteric properties. Here, we show that this mosaic, in the absence of activator, performs like a wild-type AGPase that is partially activated with 3-phosphoglyceric acid (3-PGA). In the presence of 3-PGA, enzyme properties of Mos(1-198)/SH2 are quite similar to those of the wild-type maize enzyme. In the absence of 3-PGA, however, the mosaic enzyme exhibits greater activity, higher affinity for the substrates, and partial inactivation by inorganic phosphate. The Mos(1-198)/SH2 enzyme is also more stable to heat inactivation. The different properties of this protein were mapped using various mosaics containing smaller portions of the potato small subunit. Enhanced heat stability of Mos(1-19...Continue Reading

References

May 25, 1994·Molecular & General Genetics : MGG·M J Giroux, L C Hannah
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·M J GirouxL C Hannah
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·T W GreeneT W Okita
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·T W Greene, L C Hannah
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eric D SmidanskyMichael J Giroux
May 4, 2004·Plant Physiology·Joanna M CrossL Curtis Hannah
Feb 3, 2005·The Plant Journal : for Cell and Molecular Biology·Joanna M CrossL Curtis Hannah
Feb 5, 2005·The EMBO Journal·Xiangshu JinJames H Geiger
Oct 18, 2005·Photosynthesis Research·Miguel A BallicoraJack Preiss

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Citations

Jan 8, 2009·Plant Physiology·Elizabeth A Kellogg, C Robin Buell
Nov 6, 2009·Plant Physiology·Susan K BoehleinJon D Stewart
Feb 9, 2010·Eukaryotic Cell·Randor RadakovitsMatthew C Posewitz
Jul 5, 2013·PloS One·Carlos M FigueroaAlberto A Iglesias

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