Characterization of an ecto-ATPase activity in Fonsecaea pedrosoi

Archives of Microbiology
Italo Collopy-JuniorJosé Roberto Meyer-Fernandes

Abstract

In this work, we characterized an ecto-ATPase activity in intact mycelial forms of Fonsecaea pedrosoi, the primary causative agent of chromoblastomycosis. In the presence of 1 mM EDTA, fungal cells hydrolyzed adenosine-5'-triphosphate (ATP) at a rate of 84.6 +/- 11.3 nmol Pi h(-1) mg(-1) mycelial dry weight. The ecto-ATPase activity was increased at about five times (498.3 +/- 27.6 nmol Pi h(-1) mg(-1)) in the presence of 5 mM MgCl2, with values of Vmax and apparent Km for Mg-ATP(2-) corresponding to 541.9 +/- 48.6 nmol Pi h(-1) mg(-1) cellular dry weight and 1.9 +/- 0.2 mM, respectively. The Mg2+-stimulated ecto-ATPase activity was insensitive to inhibitors of intracellular ATPases such as vanadate (P-ATPases), bafilomycin A1(V-ATPases), and oligomycin (F-ATPases). Inhibitors of acid phosphatases (molybdate, vanadate, and fluoride) or alkaline phosphatases (levamizole) had no effect on the ecto-ATPase activity. The surface of the Mg2+ -stimulated ATPase in F. pedrosoi was confirmed by assays in which 4,4'-diisothiocyanostylbene-2,2'-disulfonic acid (DIDS), a membrane impermeant inhibitor, and suramin, an inhibitor of ecto-ATPase and antagonist of P2 purinoreceptors. Based on the differential expression of ecto-ATPases in the d...Continue Reading

References

Feb 1, 1991·Current Opinion in Immunology·T H Steinberg, F Di Virgilio
Oct 1, 1988·Archives of Biochemistry and Biophysics·J R Meyer-Fernandes, A Vieyra
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E J BowmanK Altendorf
Dec 1, 1986·Journal of General Microbiology·W N ArnoldP D Coleman
Jan 1, 1995·International Review of Cytology·L Plesner
Jan 1, 1995·Pflügers Archiv : European journal of physiology·A U ZiganshinG Burnstock
Jun 1, 1994·Oral Microbiology and Immunology·G D MacFarlaneM C Herzberg
Sep 1, 1993·The American Journal of Physiology·G R Dubyak, C el-Moatassim
May 15, 1993·Archives of Biochemistry and Biophysics·H B CunninghamA S Dahms
May 15, 1996·Biochemical and Biophysical Research Communications·E BarbacciE Ziparo
May 1, 1997·Archives of Biochemistry and Biophysics·J R Meyer-FernandesA H Lopes
Jan 1, 1997·Mycopathologia·E E Aquino-Piñero, N Rodríguez del Valle
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C Caruso-NevesA G Lopes
Nov 7, 1999·The Journal of Biological Chemistry·X Zhong, G Guidotti
Mar 4, 2000·Archives of Biochemistry and Biophysics·F S BarrosJ R Meyer-Fernandes
Mar 25, 2000·Journal of Leukocyte Biology·J W Goding
Mar 29, 2000·The International Journal of Biochemistry & Cell Biology·C F BernardesA E Vercesi
Apr 13, 2000·The Plant Cell·C ThomasS J Roux
May 9, 2000·Clinical and Diagnostic Laboratory Immunology·P EsterreA Andriantsimahavandy
Jun 24, 2000·European Journal of Biochemistry·R LemmensJ Sévigny
Aug 6, 2000·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·A P LemosJ R Meyer-Fernandes
Aug 24, 2000·The Journal of Biological Chemistry·X ZhongG Guidotti
Feb 24, 2001·Medical Mycology·G S De HoogF Yegres
Jun 21, 2001·Archives of Biochemistry and Biophysics·M Berrêdo-PinhoJ R Meyer-Fernandes
Sep 24, 2002·Parasitology International·José Roberto Meyer-Fernandes
Nov 21, 2002·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·José Batista de JesusJosé Roberto Meyer-Fernandes
Apr 26, 2003·The European Journal of Neuroscience·Norbert BraunHerbert Zimmermann
May 21, 2003·International Journal of Dermatology·Luiz G M CastroCarlos S Lacaz
Aug 2, 2003·International Journal of Dermatology·Marília M OgawaJane Tomimori-Yamashita
Jan 17, 2004·Medical Mycology·L F KneippJ R Meyer-Fernandes
Mar 23, 2004·Journal of the American Society of Nephrology : JASN·Bénédicte Buffin-MeyerAlain Doucet
Apr 2, 2004·Revista Do Instituto De Medicina Tropical De São Paulo·Claudio Guedes SalgadoUbirajara Imbiriba Salgado
Apr 3, 2004·Parasitology Research·José Roberto Meyer-FernandesThais Souto-Padrón
May 19, 2004·Biochemical and Biophysical Research Communications·Francesca FarinaClaudio Palleschi

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Citations

Oct 18, 2006·FEMS Yeast Research·Itallo Collopy-JuniorJosé Roberto Meyer-Fernandes
Feb 19, 2009·Acta Physiologica·G Burnstock, A Verkhratsky
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Fiona M SansomElizabeth L Hartland
Dec 21, 2010·Revista iberoamericana de micología·Gioconda San-Blas, Eva Burger
Jul 25, 2007·FEMS Microbiology Reviews·André L S SantosCeluta S Alviano
Jan 19, 2011·FEMS Microbiology Letters·Thais Russo-AbrahãoJosé Roberto Meyer-Fernandes
Nov 20, 2016·Clinical Microbiology Reviews·Flavio Queiroz-TellesThomas J Walsh
Apr 13, 2007·Archives of Microbiology·Tina Kiffer-MoreiraJosé R Meyer-Fernandes
Apr 11, 2007·FEMS Yeast Research·Tina Kiffer-MoreiraJosé Roberto Meyer-Fernandes

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