PMID: 3768396Nov 14, 1986Paper

Characterization of an endogenous inhibitor of lung 15-hydroxyprostaglandin dehydrogenase

Biochimica Et Biophysica Acta
J M Bergholte, R T Okita

Abstract

An endogenous inhibitor of the NAD+-dependent 15-hydroxyprostaglandin dehydrogenase was isolated from the 105,000 X g supernatant fraction of lungs of pregnant rabbits following DEAE chromatography. The material was heat stable and was resistant to pronase treatment. The inhibitor contained a mixture of saturated and mono-unsaturated fatty acids and cholesterol with palmitate and oleate representing the major fatty acids in the inhibitory factor. The factor inhibited prostaglandin dehydrogenase activity but had only minor effects on the activity of NAD+-dependent alcohol and lactate dehydrogenases or the NADP+-dependent isocitrate dehydrogenase. In an attempt to develop a greater understanding of the inhibitory action of fatty acids on prostaglandin dehydrogenase activity, a variety of standard fatty acids were examined for their ability to decrease enzymic activity. Oleate and palmitate inhibited enzymic activity by 70% at 10 microM, whereas arachidonate and myristate were only 30% inhibitory at this concentration. A comparison among the 18-carbon-containing fatty acids demonstrated that oleate was more potent than linoleate and linolenate in inhibiting prostaglandin dehydrogenase activity. The coenzyme A derivatives of oleate...Continue Reading

References

Oct 1, 1976·Prostaglandins·H S Hansen
Nov 1, 1976·Archives of Biochemistry and Biophysics·J Jarabak, S S Braithwaite
Mar 1, 1979·British Journal of Pharmacology·Y S BakhleP Uotila
Mar 1, 1986·Archives of Biochemistry and Biophysics·J M Bergholte, R T Okita
Feb 14, 1970·Nature·P J PiperJ H Wyllie
Sep 8, 1970·Biochimica Et Biophysica Acta·D G Ahern, D T Downing
Jul 1, 1968·Biochimica Et Biophysica Acta·C Pace-Asciak, L S Wolfe
Nov 1, 1980·American Journal of Obstetrics and Gynecology·J Jarabak
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L R Ballou, W Y Cheung
Jul 21, 2006·The Journal of Physical Chemistry. B·V B FainermanR Miller

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Citations

Apr 1, 1997·European Journal of Drug Metabolism and Pharmacokinetics·M Foldvari, C J Oguejiofor
Jul 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M MibeN Mori
Jun 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J R OkitaR T Okita
Mar 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·T OshigeN Mori
Dec 1, 1989·Prostaglandins, Leukotrienes, and Essential Fatty Acids·K NagaiN Mori

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