PMID: 6537887Jan 11, 1984Paper

Characterization of an essential disulfide bond associated with the active site of the renal brush-border membrane D-glucose transporter

Biochimica Et Biophysica Acta
R J Turner, J N George

Abstract

In a previous report (J. Biol. Chem. 258 (1983) 3565-3570) we have demonstrated that the disulfide-reducing agent dithiothreitol has two effects on the sodium-dependent outer cortical brush border membrane D-glucose transporter; the first results in a reversible increase in the affinity of the transporter for the non-transported competitive inhibitor phlorizin, while the second results in a partially reversible loss of phlorizin binding and glucose-transport activity. Evidence was presented that both of these effects are the result of the reduction of disulfide bonds on the transport molecule. In the present paper we extend our observations on the inactivation of the transporter by dithiothreitol. We provide evidence here (i) that the inactivation of the transporter by dithiothreitol is independent of the effect of the reducing agent on the affinity of the transporter, (ii) that this inactivation process is first-order in dithiothreitol and thus presumably due to the reduction of a single disulfide bond essential to the functioning of the transporter. (iii) that it is the reduction of this disulfide bond and not some subsequent conformational or other change in the transporter which results in its inactivation, (iv) that phlori...Continue Reading

References

Dec 14, 1976·Biochimica Et Biophysica Acta·M Silverman
Feb 21, 1978·Biochimica Et Biophysica Acta·R J Turner, M Silverman
Nov 1, 1966·Archives of Biochemistry and Biophysics·D F Diedrich
Mar 1, 1981·Canadian Journal of Physiology and Pharmacology·M Silverman
Jan 1, 1982·The Journal of Membrane Biology·R J Turner, A Moran
Jan 1, 1982·The Journal of Membrane Biology·R J Turner, A Moran

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Citations

Nov 1, 1989·The Journal of Membrane Biology·J N George, R J Turner
Feb 1, 2006·The Journal of General Physiology·Dominique G GagnonJean-Yves Lapointe
Aug 1, 1986·Kidney International·H Murer, P Gmaj
Jun 1, 1992·Journal of Cellular Biochemistry·M Loghman-Adham
Jan 18, 2019·Journal of Oral and Maxillofacial Pathology : JOMFP·T SmithaM A Vidya
Jul 10, 2007·The Journal of Biological Chemistry·Theeraporn PuntheeranurakRolf K H Kinne
Jan 15, 1990·Biochimica Et Biophysica Acta·M SuzukiN Yanagawa
Sep 30, 1991·Biochimica Et Biophysica Acta·B Beaumier, R Béliveau

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