Characterization of an extracellular lipase and its chaperone from Ralstonia eutropha H16

Applied Microbiology and Biotechnology
Jingnan LuAnthony J Sinskey

Abstract

Lipase enzymes catalyze the reversible hydrolysis of triacylglycerol to fatty acids and glycerol at the lipid-water interface. The metabolically versatile Ralstonia eutropha strain H16 is capable of utilizing various molecules containing long carbon chains such as plant oil, organic acids, or Tween as its sole carbon source for growth. Global gene expression analysis revealed an upregulation of two putative lipase genes during growth on trioleate. Through analysis of growth and activity using strains with gene deletions and complementations, the extracellular lipase (encoded by the lipA gene, locus tag H16_A1322) and lipase-specific chaperone (encoded by the lipB gene, locus tag H16_A1323) produced by R. eutropha H16 was identified. Increase in gene dosage of lipA not only resulted in an increase of the extracellular lipase activity, but also reduced the lag phase during growth on palm oil. LipA is a non-specific lipase that can completely hydrolyze triacylglycerol into its corresponding free fatty acids and glycerol. Although LipA is active over a temperature range from 10 °C to 70 °C, it exhibited optimal activity at 50 °C. While R. eutropha H16 prefers a growth pH of 6.8, its extracellular lipase LipA is most active between ...Continue Reading

References

Jan 1, 1992·Journal of Chemical Technology and Biotechnology·P SkagerlindK Hult
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A H HobsonD J McConnell
Aug 1, 1993·Enzyme and Microbial Technology·E J Gilbert
May 20, 1994·Journal of Molecular Biology·Y BourneC Cambillau
Aug 1, 1993·Molecular Microbiology·L G FrenkenC T Verrips
Jan 1, 1997·Methods in Enzymology·J D Schrag, M Cygler
Aug 28, 1998·Chemistry and Physics of Lipids·M LottiJ Lafuente
Sep 23, 1998·Trends in Biotechnology·K E Jaeger, M T Reetz
Sep 24, 1999·The Biochemical Journal·J L Arpigny, K E Jaeger
Dec 2, 2000·Biochimie·R Rosenstein, F Götz
Aug 9, 2001·Journal of Molecular Biology·G van PouderoyenB W Dijkstra
Mar 29, 2002·Biotechnology and Bioengineering·Marta SánchezPilar Diaz
Sep 27, 2002·Current Opinion in Biotechnology·Karl-Erich Jaeger, Thorsten Eggert
Feb 18, 2004·Applied Microbiology and Biotechnology·R GuptaP Rathi
Dec 15, 2004·Protein Expression and Purification·Dinh Thi QuyenJung-Kee Lee
Aug 20, 2005·PLoS Computational Biology·Subbulakshmi Latha CherukuvadaGautam Pennathur
May 1, 2007·Applied and Environmental Microbiology·Bouke K H L BoekemaJan Tommassen
Jun 24, 2008·Biotechnology Advances·Babu JosephGeorge Thomas
Aug 12, 2008·Advances in Colloid and Interface Science·P ReisR Miller
Sep 6, 2008·Applied Microbiology and Biotechnology·Jeong Ho JeonJung-Hyun Lee
Feb 14, 2009·Biotechnology and Bioengineering·Lene FjerbaekBirgir Norddahl
May 7, 2010·Journal of Chemical Information and Modeling·Ying WangJing-Fang Wang
Jun 11, 2010·Applied Microbiology and Biotechnology·Yung-Hun YangAnthony J Sinskey
Aug 17, 2010·Journal of Bacteriology·Christopher J BrighamAnthony J Sinskey
Feb 1, 2011·Applied Microbiology and Biotechnology·Charles F BuddeAnthony J Sinskey
Mar 1, 2011·Bioresource Technology·Etienne SéveracAlain Marty
Mar 15, 2011·Applied and Environmental Microbiology·Charles F BuddeAnthony J Sinskey

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Citations

Aug 7, 2012·Applied Microbiology and Biotechnology·Jingnan LuAnthony J Sinskey
Mar 8, 2014·Applied Microbiology and Biotechnology·Estelle GrousseauAnthony J Sinskey
Dec 18, 2013·Applied Microbiology and Biotechnology·Sebastian L RiedelChristopher J Brigham
Mar 22, 2014·Applied Microbiology and Biotechnology·Stanislav ObrucaIvana Marova
Jun 1, 2014·Applied Microbiology and Biotechnology·Toshiaki FukuiSatoshi Nakamura
Oct 3, 2015·Journal of Biotechnology·Sebastian L RiedelUlf Stahl
Aug 2, 2014·Applied Microbiology and Biotechnology·Jingnan LuAnthony J Sinskey
Aug 9, 2016·Applied and Environmental Microbiology·Chia-Suei HungWendy J Crookes-Goodson
Mar 20, 2019·Preparative Biochemistry & Biotechnology·Müslüm Altun
Jun 21, 2020·Applied and Environmental Microbiology·Christopher C AzubuikeThomas P Howard
May 26, 2018·World Journal of Microbiology & Biotechnology·Gurusamy Kutralam-Muniasamy, Fermín Peréz-Guevara
Jan 27, 2016·Journal of Bioscience and Bioengineering·Amanda C BernardiChristopher J Brigham
Aug 27, 2021·Journal of Environmental Management·Cassendra Phun Chien BongBahrul Razha Bin Chuprat

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