Characterization of an in vitro cell culture bioreactor system to evaluate anti-neoplastic drug regimens

Breast Cancer Research and Treatment
Mark N KirsteinDouglas Yee

Abstract

A dynamic 3-dimensional tissue culture system has been developed that will allow for control of gemcitabine exposure to mimic concentration-time profiles measured from biologic samples. Gemcitabine was infused into a central reservoir. Media is mixed and delivered through hollow fiber capillaries, where it diffuses into the extracapillary space containing anchorage-dependent MDA-231 cells. To test for control of gemcitabine concentration-time profiles, drug was first infused through bioreactors without cells, and gemcitabine concentrations were measured with HPLC. Concentrations could be controlled to simulate 30-min and 2.5 h infusions, and were similar in both the lumen and extracapillary space. MDA-231 cells were then seeded into control (n = 4) and gemcitabine treatment (n = 4) groups, and maintained in culture for 2 weeks. Gemcitabine (5.3 mg) was infused over 30 min to the treatment group, and blank media to the control group. Accuracy of measured gemcitabine maximum concentration (Cmax) was 83.4%, and area under the curve (AUC), 106.2%, relative to pre-experimental theoretical values. With cells present, gemcitabine AUC in the extracapillary space was 32% of the value in the lumen. For the control group, 21.2 million cel...Continue Reading

References

Mar 1, 1991·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J L AbbruzzeseM N Raber
Jan 1, 1981·European Journal of Clinical Pharmacology·M Gibaldi, J R Koup
Jul 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·P I ClarkP F Wrigley
Feb 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A de GramontM Krulik
May 20, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M MarkmanW P McGuire
Oct 3, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C TolisG Giaccone
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J R KroepG J Peters
Jun 30, 2000·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·D A Rew, G D Wilson
Jul 15, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A P VenookM J Ratain
Oct 6, 2000·Computers and Biomedical Research, an International Journal·C FarencF Bressolle
Jul 4, 2001·International Journal of Cancer. Journal International Du Cancer·P CappellaP Ubezio
Jan 18, 2002·Antimicrobial Agents and Chemotherapy·G L DrusanoJ A Bilello
May 10, 2002·Breast Cancer Research and Treatment·Daigo YamamotoKoshiro Hioki
Jul 30, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Margaret TemperoJames Abbruzzese
Oct 25, 2003·International Journal of Radiation Oncology, Biology, Physics·Bea PauwelsFilip Lardon
Jan 1, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Baruch BrennerDavid P Kelsen
Aug 18, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Clinton F StewartAmar Gajjar
Oct 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marie SuggittMichael C Bibby
Dec 9, 2004·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Nicole M ChandlerMark P Callery
Mar 17, 2005·Breast Cancer Research and Treatment·Shadan AliPhilip A Philip

❮ Previous
Next ❯

Citations

Apr 13, 2007·Cancer Chemotherapy and Pharmacology·Mark N KirsteinDouglas Yee
Nov 29, 2007·Anti-cancer Drugs·Mark N KirsteinTodd M Tuttle
Mar 30, 2010·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dorota BartusikGino Fallone
Jun 19, 2008·Journal of Bioscience and Bioengineering·Gianni CiofaniPaolo Dario
Jul 27, 2007·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Mark N KirsteinRobert A Kratzke
Apr 6, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mark N KirsteinRory P Remmel
Feb 22, 2008·Journal of Biomedicine & Biotechnology·George A ThouasKerry Hourigan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.