Characterization of an industry-grade CMOS camera well suited for single molecule localization microscopy - high performance super-resolution at low cost

Scientific Reports
Robin DiekmannThomas Huser

Abstract

Many commercial as well as custom-built fluorescence microscopes use scientific-grade cameras that represent a substantial share of the instrument's cost. This holds particularly true for super-resolution localization microscopy where high demands are placed especially on the detector with respect to sensitivity, noise, and also image acquisition speed. Here, we present and carefully characterize an industry-grade CMOS camera as a cost-efficient alternative to commonly used scientific cameras. Direct experimental comparison of these two detector types shows widely similar performance for imaging by single molecule localization microscopy (SMLM). Furthermore, high image acquisition speeds are demonstrated for the CMOS detector by ultra-fast SMLM imaging.

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Citations

Jan 10, 2019·PloS One·Benedict DiederichRainer Heintzmann
Jan 1, 2020·PloS One·Benedict DiederichRainer Heintzmann
Jan 5, 2020·Nature Communications·Biagio MandracchiaShu Jia
Mar 19, 2019·Annual Review of Physical Chemistry·Joshua D Morris, Christine K Payne
Aug 9, 2019·Nature Communications·Koen J A MartensJohannes Hohlbein
Mar 3, 2021·Optics Letters·Oleksii Matsiaka, Taras Plakhotnik

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Methods Mentioned

BETA
fluorescence microscopy
chip
chips
super-resolution microscopy
Fluorescence

Software Mentioned

Windows 7
sCMOS
Micro Manager
Fiji
ThunderSTORM
SMLM
IDS Micro Manger
MicroManager
Matlab

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