PMID: 11333304May 3, 2001Paper

Characterization of an isoform of rice starch branching enzyme, RBE4, in developing seeds

Plant & Cell Physiology
K MizunoT Baba

Abstract

cDNA clones encoding an isoform of starch branching enzyme, RBE4, have been identified from a developing rice seed cDNA library, using a synthetic oligonucleotide probe corresponding to the N-terminal amino acid sequence of RBE4. The cDNA-derived amino acid sequence indicated that RBE4 is initially produced as a precursor protein of 841 amino acids, including a 53-residue transit peptide at the N-terminus. The mature form of RBE4 shared a high degree of sequence identity (80%) with mature RBE3, and possessed an N-terminal extra sequence, as found in RBE3. Northern blot analysis demonstrated that the RBE4 gene is expressed in both leaves and developing seeds. The RBE4 gene was distinguished from the RBE1 and RBE3 genes by expression at the earlier stages of seed development. To examine enzymatic functions of RBE4, recombinant proteins were produced in Escherichia coli cells, and purified by two chromatographic separations. The branched alpha-glucans produced by the recombinant enzymes from potato amylose revealed the different patterns of oligosaccharide chain transfer. The peak of major branches of the products by RBE3 or RBE4 was 6 glucose units, whereas the peaks of major branches of the products by RBE1 were 6 and 11 glucose...Continue Reading

Citations

May 20, 2003·Current Opinion in Plant Biology·Martha G JamesAlan M Myers
May 16, 2012·Molecular Biology and Evolution·Chun LiYuan-Ming Zhang
Apr 22, 2009·Plant & Cell Physiology·Takayuki SawadaYasunori Nakamura
Mar 23, 2010·Plant & Cell Physiology·Yasunori NakamuraShinichi Kitamura
Sep 10, 2014·IUBMB Life·Ian J Tetlow, Michael J Emes
Nov 11, 2008·Bioscience, Biotechnology, and Biochemistry·Nhuan Thi VuMakoto Kimura
Apr 25, 2007·Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology·Jun-Jie ZhangYu-Bi Huang
Nov 9, 2005·Journal of Experimental Botany·Takashi OhdanYasunori Nakamura
Nov 25, 2003·Plant & Cell Physiology·Kouichi MizunoTatsuhito Fujimura
Sep 9, 2010·Journal of Agricultural and Food Chemistry·Jia-Wei ChangYuan-Tih Ko

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