Characterization of an N-system amino acid transporter expressed in retina and its involvement in glutamine transport

The Journal of Biological Chemistry
S GuJ X Jiang

Abstract

We report here on the characterization of a mouse N-system amino acid transporter protein, which is involved in the transport of glutamine. This protein of 485 amino acids shares 52% sequence homology with an N-system amino acid transporter, mouse N-system amino acid transporter (mNAT) and its orthologs. Because this protein shares a high degree of sequence homology and functional similarity to mNAT, we named it mNAT2. mNAT2 is predominately expressed in the retina and to a slightly lesser extent in the brain. In the retina, it is located in the axons of ganglion cells in the nerve fiber layer and in the bundles of the optic nerve. Functional analysis of mNAT2 expressed in Xenopus oocytes revealed that the strongest transport activities were specific for l-glutamine. In addition, mNAT2 is a Na(+)- and pH-dependent, high affinity transporter and partially tolerates substitution of Na(+) by Li(+). Additionally, mNAT2 functions as a carrier-mediated transporter that facilitates efflux. The unique expression pattern and selective glutamine transport properties of mNAT2 suggest that it plays a specific role in the uptake of glutamine involved in the generation of the neurotransmitter glutamate in retina.

References

Oct 1, 1978·Journal of Neurochemistry·M J VoadenB Morjaria
May 28, 1979·Biochemical and Biophysical Research Communications·M S KilbergM E Handlogten
Jan 1, 1990·Experimental Physiology·G A Barker, J C Ellory
Jan 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H J BurgerD Mecke
Dec 1, 1989·The Journal of Membrane Biology·P M TaylorM J Rennie
Nov 1, 1974·Nature·A J KennedyJ Marshall
Sep 1, 1983·Journal of Neurochemistry·C M ThankiH F Bradford
Sep 8, 1995·Cell·P Camacho, J D Lechleiter
Apr 1, 1995·Developmental Biology·J X JiangD A Goodenough
Mar 1, 1994·Molecular Biology of the Cell·J X JiangD L Paul
Jun 21, 1996·The Journal of Biological Chemistry·N Utsunomiya-TateY Kanai
Jan 1, 1996·Annual Review of Biochemistry·M S Malandro, M S Kilberg
Dec 1, 1996·Cell and Tissue Research·T RauenH Wässle
Aug 29, 1998·The Journal of Cell Biology·L M JohnP Camacho
Oct 28, 1998·Physiological Reviews·M PalacínA Zorzano
Oct 26, 1999·Progress in Retinal and Eye Research·M Kalloniatis, G Tomisich
Feb 8, 2000·The Journal of Biological Chemistry·H VaroquiJ D Erickson
May 17, 2000·The Journal of Biological Chemistry·D YaoJ D Erickson
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·R J ReimerR H Edwards

❮ Previous
Next ❯

Citations

Dec 18, 2001·Journal of Neuroscience Research·A M BrownB R Ransom
May 14, 2003·Neurochemistry International·Monika DolińskaJan Albrecht
Apr 23, 2011·The Journal of Biological Chemistry·Maria G A HägglundRobert Fredriksson
Sep 16, 2011·The Journal of Biological Chemistry·Qian ShiJean X Jiang
Mar 7, 2002·Current Opinion in Ophthalmology·Francine B Wein, Leonard A Levin
Nov 4, 2004·Biochemical and Biophysical Research Communications·Martin AkermanDan Zilberstein
Dec 3, 2014·Journal of Molecular Biology·Maria G A HägglundRobert Fredriksson
May 16, 2009·Neuroscience·C ErnstG Turecki
Nov 18, 2005·Advanced Drug Delivery Reviews·Patrick M HughesClive G Wilson
Sep 23, 2016·The Biochemical Journal·Rugmani Padmanabhan IyerJean X Jiang
Sep 28, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel A SáenzRuth E Rosenstein
Mar 28, 2017·Experimental Biology and Medicine·Andrew SoperKei Sato
Aug 7, 2003·American Journal of Physiology. Endocrinology and Metabolism·Fanfan ZhouGuofeng You
May 19, 2005·The Journal of Biological Chemistry·Sumin GuJean X Jiang

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.