Characterization of an oxaloacetate decarboxylase that belongs to the malic enzyme family

FEBS Letters
Pablo D SenderC Magni

Abstract

The citM gene from Lactococcus lactis CRL264 was demonstrated to encode for an oxaloacetate decarboxylase. The enzyme exhibits high levels of similarity to malic enzymes (MEs) from other organisms. CitM was expressed in Escherichia coli, purified and its oxaloacetate decarboxylase activity was demonstrated by biochemical and genetic studies. The highest oxaloacetate decarboxylation activity was found at low pH in the presence of manganese, and the Km value for oxaloacetate was 0.52+/-0.03 mM. However, no malic activity was found for this enzyme. Our studies clearly show a new group of oxaloacetate decarboxylases associated with the citrate fermentation pathway in gram-positive bacteria. Furthermore, the essential catalytic residues were found to be conserved in all members of the ME family, suggesting a common mechanism for oxaloacetate decarboxylation.

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Citations

Nov 10, 2011·The Journal of Microbiology·Baojuan WangGuoping Zhu
May 28, 2013·Applied and Environmental Microbiology·Pablo MorteraJuke S Lolkema
Feb 5, 2008·Applied and Environmental Microbiology·Nieves García-QuintánsPaloma López
Feb 26, 2013·Applied and Environmental Microbiology·Guillermo D RepizoChristian Magni
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Iwona Sobczak, Juke S Lolkema
Nov 26, 2015·International Journal of Food Microbiology·Gabriela P MartinoChristian Magni
Dec 18, 2013·The Biochemical Journal·Wenhe ZhongMalcolm D Walkinshaw
Aug 17, 2005·FEMS Microbiology Reviews·Uwe Sauer, Bernhard J Eikmanns
Mar 1, 2018·Biochemical Society Transactions·Alexander K H WeissPidder Jansen-Dürr
Dec 9, 2020·FEMS Microbiology Reviews·Jeroen G KoendjbiharieServé W M Kengen
Feb 25, 2021·Nature Communications·Christopher John HardingAndrew Lee Lovering
May 7, 2021·FEMS Microbiology Reviews·Jeroen G KoendjbiharieServé W M Kengen

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