PMID: 90522Sep 27, 1979

Characterization of an RNA-directed DNA-polymerase from a cell line derived from a radiation-induced lymphoma in mice

Biochimica Et Biophysica Acta
P SarinR C Gallo

Abstract

An RNA-directed DNA polymerase was purified from a cell line derived from a radiation-induced lymphoma in NIH Swiss mice which produced non-infectious type C virus particles. The enzyme was isolated from a high speed particulate fraction which bands at a density of 1.16--1.19 g/ml in a sucrose gradient, and purified by successive chromatography on DEAE-cellulose, phosphocellulose and hydroxyapatite. The purified DNA polymerase has a molecular weight of 68 000, a pH optimum of 7.5, a KCl optimum of 50 mM, and a Mn2+ optimum of 0.25 mM. It prefers (dT)15 . (A)n to (dT)15 . (dA)n as the primer template and transcribes the poly(C) strand of (dG)15 .(C)n and (dG)15 . (OMeC)n. It transcribes heteropolymeric regions of avian myeloblastosis virus 70 S RNA, and is inhibited by antiserum to Rauscher murine leukemia virus DNA polymerase. Comparison of the properties of DNA polymerase purified from radiation-induced lymphoma cells with the DNA polymerase purified from non-defective murine type C RNA tumor viruses shows that the mouse lymphoma enzyme is both biochemically and immunologically related to murine leukemia virus DNA polymerases.

Citations

Jun 27, 1980·Biochimica Et Biophysica Acta·P SarinB Friedman
May 15, 1982·International Journal of Cancer. Journal International Du Cancer·P SarinR C Gallo

Related Concepts

Metazoa
IgG2B
Immunoassay Method
Lymphoma
Manganese
Radiation-Induced Cancer
Slow-K
RNA-Directed DNA Polymerase
Genetic Template
Mouse, Swiss

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