PMID: 6540339Jul 1, 1984Paper

Characterization of an unusual sex steroid binding component from the chicken oviduct

Journal of Steroid Biochemistry
M F Ruh, D O Toft

Abstract

In addition to the classical estrogen receptor, chick oviduct cytosol contains a sex steroid binding component (SSB) with specificity for steroidal estrogens, androgens and progestins. We have optimized the measurement of SSB and have further characterized this protein. It was possible to quantitate [3H]estradiol binding to SSB by performing the measurements in the presence of excess diethylstilbestrol, which saturates the estrogen receptor and does not bind to SSB, and by using excess progesterone to determine nonspecific binding. Since SSB appears to be quite unstable with rapid hormone dissociation kinetics, we determined that short incubation times (usually 2 h) at 0 degrees C with 20-30% glycerol in the buffer gave optimal SSB measurements. The affinity of SSB for estradiol (Kd = 20 nM) is about 5% that of the estrogen receptor. In addition to estradiol, several androgens and progestins bind to SSB. However, the nonsteroidal antiestrogen, H1285 does not bind to SSB even though it binds well to the avian estrogen receptor. The tissue content of SSB is about 15-fold greater than for estrogen receptor and is stimulated by estrogen treatment. Whereas labeled SSB cannot be readily resolved by ion-exchange chromatography due to ...Continue Reading

References

Jan 1, 1975·Endocrinology·R W Harrison, D O Toft
Sep 15, 1975·European Journal of Biochemistry·M Best-BelpommeE E Baulieu
Mar 15, 1978·Biochemical and Biophysical Research Communications·H ErikssonJ H Clark
Jan 1, 1968·The Journal of Clinical Endocrinology and Metabolism·S G Korenman
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R N Taylor, R G Smith
Jan 8, 1980·Biochemistry·H Nishigori, D Toft

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Citations

Feb 1, 1989·Biulleten' eksperimental'noĭ biologii i meditsiny·A N Smirnov, T A Shchelkunova
Dec 1, 1989·Journal of Steroid Biochemistry·D L Ellis, B J Danzo
Dec 1, 1988·General and Comparative Endocrinology·K W Selcer, W W Leavitt

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