Characterization of and application of monoclonal antibodies against Rickettsia africae, a newly recognized species of spotted fever group rickettsia.

Journal of Clinical Microbiology
W XuD Raoult

Abstract

Rickettsia africae is a newly described species which causes African tick bite fever. Mediterranean spotted fever caused by R. conorii is endemic in the same regions of Africa as tick bite fever, and differentiation of the two syndromes by characterization of their etiological agents is important for epidemiological studies. R. africae and R. conorii are, however, difficult to distinguish, and therefore, our aim was to produce monoclonal antibodies to address this problem. Monoclonal antibodies were produced against R. africae by fusing splenocytes from BALB/C mice immunized with purified rickettsial organisms and SP2/0-Ag14 myeloma cells. A total of 355 hybridomas producing monoclonal antibodies to R. africae were identified by initial screening with six different antigens by microimmunofluorescence assay. A panel of 23 representative monoclonal antibodies were selected and subcloned. This panel was screened with a further 17 different spotted fever group (SFG) rickettsial reference antigens. Of these 23 monoclonal antibodies, 1 cross-reacted with only R. parkeri, whereas the others cross-reacted with more than two different antigens. Immunoblotting indicated that all the monoclonal antibodies were directed against the epitope...Continue Reading

References

Jul 1, 1992·The American Journal of Tropical Medicine and Hygiene·D H WalkerH M Feng
Apr 1, 1992·International Journal of Systematic Bacteriology·T UchidaD H Walker
Sep 1, 1991·Journal of Medical Entomology·P J Kelly, P R Mason
May 1, 1991·Transactions of the Royal Society of Tropical Medicine and Hygiene·P J KellyP R Mason
Oct 1, 1990·Journal of Clinical Microbiology·P J Kelly, P R Mason
Nov 1, 1988·The American Journal of Tropical Medicine and Hygiene·M Y FanD H Walker
Jun 1, 1989·The Journal of Infectious Diseases·T UchidaD H Walker
Feb 1, 1989·The American Journal of Tropical Medicine and Hygiene·M Marrero, D Raoult
Oct 1, 1988·Journal of Clinical Microbiology·J E McDadeC L Spruill
Sep 1, 1988·The Journal of Infectious Diseases·T UchidaA H Koyama
Jun 1, 1985·The Journal of Infectious Diseases·R L AnackerL A Thomas
Nov 1, 1984·Infection and Immunity·J V Lange, D H Walker
Mar 1, 1994·The American Journal of Tropical Medicine and Hygiene·T BabalisD Raoult
Jul 1, 1993·International Journal of Systematic Bacteriology·L BeatiD Raoult
Oct 1, 1993·International Journal of Systematic Bacteriology·L Beati, D Raoult
Jun 1, 1995·Research in Microbiology·V Roux, D Raoult
Apr 1, 1996·International Journal of Systematic Bacteriology·P J KellyD Raoult
May 1, 1964·Stain Technology·D F GIMENEZ

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Citations

Mar 19, 1999·Emerging Infectious Diseases·P E FournierD Raoult
Mar 15, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Parola, D Raoult
Mar 11, 2003·Clinical and Diagnostic Laboratory Immunology·Rong Fang, Didier Raoult
Oct 23, 1997·Clinical Microbiology Reviews·D Raoult, V Roux
Aug 31, 2002·Journal of Clinical Microbiology·Rong FangDidier Raoult

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