PMID: 3748895Jan 1, 1986Paper

Characterization of androgen receptors in normal and malignant human prostatic tissues

The Prostate
F RadwanJ G Lehoux

Abstract

The aim of this work was to study the physico-chemical characteristics of androgen receptors of normal and malignant human prostatic tissues. Association (k+1) and dissociation (k-1) rate constants and sedimentation profiles on sucrose density gradients were determined on [3H]androgen receptor complexes. In the presence of 20 mM sodium molybdate, no significant difference in k+1 and k-1 values could be found between cytosolic receptor preparations from normal and malignant specimens. The values obtained for k+1 (mean +/- SD) were 5 +/- 2 X 10(6)M-1 min-1 (N = 3) and 5 +/- 2 X 10(6)M-1 (N = 5); k-1 values of 23 +/- 4 X 10(-4) min-1 (N = 4) and 25 +/- 3 X 10(-4) min-1 (N = 5) were obtained for normal and malignant tissues respectively. Similar Nmax values were also obtained for normal [(mean +/- SD) 26 +/- 10 fmol/mg cytosolic protein (N = 5)] and malignant [20 +/- 9 fmol/mg protein (N = 7)] tissues. A statistically significant difference was found however, between k-1 values measured on [3H]androgen receptor complexes of nuclear extracts; values of 15 +/- 3 X 10(-4) min-1 (N = 4) and 9 +/- 1 X 10(-4) min-1 (N = 6) were found for normal and malignant tissue preparations respectively. This was also accompanied by a higher level of...Continue Reading

References

Oct 1, 1975·The Journal of Clinical Endocrinology and Metabolism·V RosenP Robel
Jan 1, 1975·Vitamins and Hormones·A AttramadalO Torgersen
Jan 1, 1979·The Journal of Urology·G Lieskovsky, N Bruchovsky
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Jan 1, 1984·The Journal of Clinical Endocrinology and Metabolism·J GellerD A Nachtsheim
Jun 1, 1984·Journal of Steroid Biochemistry·J C Chapman, A I Frankel

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Citations

Jan 1, 1988·Journal of Steroid Biochemistry·F RadwanJ G Lehoux
Jan 1, 1989·Journal of Receptor Research·M Nijs, A Coune
Mar 11, 1992·Critical Reviews in Oncology/hematology·D N Danforth

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