Characterization of anti-apolipoprotein A-I monoclonal antibodies and their use in the measurement of apolipoprotein A-I by a two-site enzyme immunoassay.

Journal of Immunological Methods
D Y Dubois, C L Malmendier

Abstract

Six monoclonal antibodies raised against pure apo A-I or high density lipoprotein were characterized for epitope specificity by enzyme and radioimmunoassays, immunodiffusion and affinity chromatography. The six antibodies were classified into three groups according to the region of apo A-I they reacted with. The antibody VI10H, from group II, appeared to recognize a region fully exposed on native HDL-apo A-I, whereas group I comprised antibodies specific for a partially masked region. Group III comprised only one antibody. Use of the non-ionic detergent Tween 20 in the immunoassays permitted antibodies from the three groups to react with their respective epitope on native HDL-apo A-I. An antibody from group I (V4F) was chosen as the first antibody and VI10H, the antibody showing the highest affinity, was chosen for the anti-A-I-peroxidase conjugate in a two-site enzyme immunoassay.

References

Dec 21, 1977·Biochimica Et Biophysica Acta·J ShepherdE Farish
Dec 1, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·C L MalmendierC Delcroix
Dec 1, 1987·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·C BetardJ C Fruchart
Jan 1, 1988·Atherosclerosis·S E Olpin, C P Price
Aug 9, 1988·Journal of Immunological Methods·D Y Dubois, C L Malmendier
Apr 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·E KorenP Alaupovic
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger
Sep 1, 1981·Journal of Virological Methods·I K Mushahwar, L R Overby
Dec 5, 1980·Biochimica Et Biophysica Acta·S J Mao, B A Kottke

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