PMID: 9661816Jul 14, 1998Paper

Characterization of anti-mouse Fc gamma RII single-chain Fv fragments derived from human phage display libraries

Immunotechnology : an International Journal of Immunological Engineering
A M McCallL M Weiner

Abstract

Few antibodies are available to study the function of the Fc gamma RII murine immunoglobulin receptor. Human phage display libraries represent a potential source of single-chain Fv (sFv) to facilitate the study of the Fc gamma RII murine immunoglobulin receptor. To isolate human sFv specific for mouse Fc gamma RII. Two human phage display libraries were selected for reactivity to mouse Fc gamma RII. Those human anti-mouse Fc gamma RII sFv that were derived from the libraries were characterized with respect to kinetics, cellular binding, epitope specificity and amino acid sequence. Nine anti-mouse Fc gamma RII sFv molecules were isolated from two human phage display libraries (Marks et al., J Mol Biol 1991;222:581-597; Sheets et al., Proc Natl Acad Sci USA, in press). Surface plasmon resonance (SPR) analysis revealed that the human anti-mouse Fc gamma RII sFv had off-rates ranging from 10(-2) to 10(-3) s-1, with KD values calculated to range between 10(-7) and 10(-9) M. The binding of the FITC-labeled human anti-mouse Fc gamma RII sFv to mouse peritoneal neutrophils was not detected by flow cytometry, due to the rapid off-rates of these monomeric proteins. However, when the human anti-mouse Fc gamma RII sFv were coated on yellow...Continue Reading

References

Dec 5, 1991·Journal of Molecular Biology·J D MarksG Winter
Jan 1, 1991·Annual Review of Immunology·J V Ravetch, J P Kinet
Aug 1, 1990·The Journal of Experimental Medicine·T J Vasicek, P Leder
May 1, 1990·The Journal of Experimental Medicine·P BorosJ C Unkeless
Mar 1, 1988·The Journal of Cell Biology·F Di VirgilioS C Silverstein
Apr 1, 1986·The Journal of Experimental Medicine·P UkkonenI Mellman
Dec 9, 1994·Journal of Chromatography. B, Biomedical Applications·C SautèsW H Fridman
Jul 1, 1993·European Journal of Immunology·S C Williams, G Winter
Apr 1, 1994·European Journal of Immunology·J P CoxG Winter

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Citations

Dec 28, 1999·Journal of Molecular Recognition : JMR·D G Myszka
Oct 17, 2001·Experimental Hematology·B P Mullaney, M G Pallavicini
Dec 1, 2006·American Journal of Pharmaceutical Education·Lisa M GuirguisNathan L Kanous
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A M McCallL M Weiner

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