Characterization of anti-phosphatidylcholine polyreactive natural autoantibodies from normal human subjects

Journal of Autoimmunity
Javier CabiedesD Alarcón-Segovia

Abstract

In 1990 our group reported a patient with autoimmune hemolytic anemia and high titers of IgM anticardiolipin antibodies that cross-reacted with phosphatidylcholine (PTC). These autoantibodies also recognized bromelain-treated erythrocytes (BrE) and in vitro aged erythrocytes. The epitope exposed with this treatment is PTC. To detect and characterize antiphosphatidylcholine antibodies (anti-PTC) in a normal human population, we studied by ELISA the presence of serum anti-PTC (IgG and IgM) in clinically healthy human subjects. The most representative samples were also studied for IgG or IgM activity against BrE by flow cytometry, rheumatoid factor activity, anti-dsDNA, anti-ssDNA by ELISA and by indirect immunofluorecence (IIF) using HEp-2 line and a healthy human fibroblast strain as substratum. Eighty five percent of sera had IgM anti-PTC and none had IgG. IgM antibodies against BrE were inhibited by PTC micelles (mPTC). Anti-PTC were also inhibited by phosphorylcholine and phosphatidic acid. Aggregated gammaglobulin (AGG) reactivity was inhibited by dsDNA and mPTC. The IgM anti-dsDNA activity was inhibited by soluble dsDNA, AGG and mPTC. All sera gave intermediate filaments pattern by IIF and reacted against purified vimentin ...Continue Reading

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Citations

Apr 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shih-Shih ChenNicholas Chiorazzi
Jan 22, 2009·Journal of Neuroinflammation·Lawrence L HorstmanAlireza Minagar
Sep 25, 2010·Seminars in Cancer Biology·Anders RosénRichard Rosenquist
Aug 21, 2015·Annals of the New York Academy of Sciences·Rebecca A LeeXuemei Zhong
Jan 30, 2008·Vaccine·Carl R Alving, Mangala Rao
Sep 25, 2007·Journal of Autoimmunity·Ben RowleyRichard R Hardy
Apr 29, 2006·Cellular Immunology·Caroline L F Ciurana, C Erik Hack
Aug 9, 2013·Blood·Vipul ShuklaRunqing Lu
May 3, 2008·Nature Protocols·Silvia S Pierangeli, Eon Nigel Harris
Dec 27, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew ElvingtonStephen Tomlinson
Jun 13, 2020·International Journal of Molecular Sciences·Paul R J AmesFabrizio Gentile
Oct 20, 2019·Biomedical Journal·Simon Fillatreau
Apr 12, 2003·Progress in Lipid Research·John A McIntyreW Page Faulk

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