Abstract
A previous study has characterized the major 47 kDa anti-sticking factor (ASF-I) from goat cauda-epididymal plasma (Roy, N., and Majumder, G.C., Biochim. Biophys. Acta, 991:114-122, 1989). This study reports the purification and characterization of ASF-II, another anti-sticking factor from the goat epididymal plasma. ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques. It showed a single protein band by both non-denaturing and SDS-polyacrylamide gel electrophoresis. ASF-II showed a molecular weight of 36,000 and a sedimentation constant of 2.4S. ASF-II is largely stable to heat treatment and it is a specific glycoprotein having high affinity and specificity for its anti-sticking action. At saturating concentration (1 nM) it inhibited adhesion of nearly 50% of spermatozoa to the glass surface of the haemocytometer counting chamber. Both the protein and sugar parts of the factor are essential for the anti-sticking activity since it lost its activity completely when treated with trypsin, L-fucosidase, or mannosidase. ASF-II does not coat the glass surface and it binds to spermatozoa. Data are...Continue Reading
References
Jul 1, 1979·Fertility and Sterility·B E MortonR Sagadraca
Dec 1, 1977·Fertility and Sterility·J E SokoloskiD P Wolf
Sep 1, 1978·Journal of Reproduction and Fertility·B Müller, C Kirchner
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Apr 25, 1989·Biochimica Et Biophysica Acta·N Roy, G C Majumder
Jan 1, 1986·Journal of Cell Science. Supplement·D R Garrod
Mar 1, 1985·Andrologia·N RoyC K Chakrabarti
Feb 1, 1971·Journal of Reproduction and Fertility·Z Morita, M C Chang
Nov 1, 1973·Journal of Reproduction and Fertility·B Morton, T S Chang
Feb 7, 1966·Biochimica Et Biophysica Acta·L M Siegel, K J Monty
Aug 15, 1970·Nature·U K Laemmli
Sep 1, 1980·Fertility and Sterility·R D AmelarC Schoenfeld
Sep 1, 1983·Andrologia·D P Chaudhuri, G C Majumder
Jan 1, 1984·Journal of Reproduction and Fertility·G C Majumder, C K Chakrabarti
Sep 15, 1982·Analytical Biochemistry·M A Markwell
Dec 1, 1981·Biology of Reproduction·D T StephensD D Hoskins
Sep 1, 1980·Biology of Reproduction·D V Brown, P L Senger
May 1, 1964·Biochemistry·G K ACKERS