Abstract
The aim of this study was to evaluate seven anti-TIMP-1 (tissue inhibitor of metalloproteinase-1) monoclonal antibodies by immunohistochemical (IHC) staining of formalin-fixed, paraffin-embedded (FFPE) tissue. Detection of the TIMP-1 protein was studied by IHC in FFPE human archival normal and neoplastic samples. Indirect IHC technique was used, and the seven antibodies (clones VT1, VT2, VT4, VT5, VT6, VT7, and VT8) were tested in various concentrations using different pretreatment protocols. All seven VT antibodies specifically immunostained the cytoplasm of islets of Langerhans cells in normal pancreas, epithelial cells of hyperplastic prostate, tumor cells of medullary thyroid carcinoma, and fibroblast-like cells of malignant melanoma. Specificity of the anti-TIMP-1 antibodies was confirmed by several controls, e.g., Western blotting on proteins extracted from FFPE tissue showed that the VT7 antibody reacted specifically with a protein band of approximately 28 kDa, corresponding to the molecular mass of TIMP-1. However, sensitivity varied with the different antibodies. Use of heat-induced epitope retrieval (HIER) and the VT7 clone applied at low concentrations demonstrated more intense immunoreactivity with the TIMP-1-positi...Continue Reading
References
Aug 1, 1992·The Journal of Cell Biology·C M Alexander, Z Werb
Nov 11, 1991·International Journal of Cancer. Journal International Du Cancer·R E HewittD R Turner
Feb 17, 1989·Science·R KhokhaD T Denhardt
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F CarmichaelG P Stricklin
Sep 15, 1986·The Biochemical Journal·T E CawstonB Hazleman
Oct 1, 1996·Japanese Journal of Clinical Oncology·M MurashigeM Kobayashi
Nov 1, 1996·Diseases of the Colon and Rectum·T Tomita, K Iwata
Mar 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiC R Taylor
Aug 1, 1997·Histopathology·T Tomita
Apr 29, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K IkedaM Monden
Dec 3, 1998·The Journal of Clinical Investigation·L GuedezM Stetler-Stevenson
Jun 9, 1999·British Journal of Cancer·K AirolaU K Saarialho-Kere
Jul 17, 1999·British Journal of Cancer·M N Holten-AndersenR W Stephens
Nov 5, 1999·The Journal of Pathology·J L JonesR A Walker
Nov 5, 1999·The Journal of Pathology·S Curran, G I Murray
Mar 10, 2000·Biochimica Et Biophysica Acta·K BrewH Nagase
Mar 10, 2001·Clinical & Experimental Metastasis·G FassinaA Albini
Jul 18, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiC R Taylor
Jan 18, 2002·The Journal of Biological Chemistry·Frank R MurphyJohn P Iredale
Jan 1, 1997·Endocrine Pathology·Tatsuo Tomita
Feb 28, 2003·Human Pathology·Daniela MassiMarco Santucci
Mar 18, 2003·The Biochemical Journal·Elise LambertEmmanuelle Petitfrere
Aug 9, 2003·The Journal of Biological Chemistry·Xu-Wen LiuHyeong-Reh Choi Kim
Oct 31, 2003·Expert Reviews in Molecular Medicine·Marc A LafleurDylan R Edwards
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Mads N Holten-AndersenBoye Schnack Nielsen
Dec 14, 2004·Biochimica Et Biophysica Acta·Olaf R F MookCornelis J F Van Noorden
Dec 14, 2004·The Journal of Urology·Alvin Y LiuDeborah L Diamond
May 5, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Nanna Møller SørensenGerard J Davis
Jun 11, 2005·Endocrine-related Cancer·S Ø WürtzN Brünner
Citations
Jun 3, 2014·BMC Cancer·Charlotte Levin Tykjær JørgensenDorte L Nielsen
May 13, 2010·BMC Cancer·Karina Dahl SteffensenAnders Jakobsen
Dec 14, 2011·Breast Cancer Research and Treatment·Pernille Braemer HertelTorsten O Nielsen
Jan 22, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiaohong DengAnne-Sofie Schrohl
Jul 25, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Christina BjerreJan Stenvang
Apr 11, 2013·Breast Cancer Research : BCR·Alison F MunroJohn M S Bartlett
Sep 29, 2011·Archives of Medical Research·Ju-Han LeeYoung-Sik Kim
Aug 31, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Søren Astrup JensenJens Benn Sørensen
Jun 19, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Gro L WillemoeNils Brünner
Sep 11, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sanne Kjaer-FrifeldtAnders Jakobsen
Nov 17, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Tove Filtenborg TvedskovNils Brünner
Oct 15, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Lars Holten-AndersenJesper Reibel
Jan 7, 2015·The Prostate·Yixuan GongWilliam K Oh
Jan 16, 2013·International Journal of Pediatric Otorhinolaryngology·Marcin MusiatowiwczStanisław Sulkowski
Sep 14, 2016·Journal of Neuro-oncology·Charlotte Aaberg-JessenBjarne W Kristensen
Apr 21, 2010·Neurosurgery·Karina ChristensenBjarne Winther Kristensen
Dec 30, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Bent EjlertsenNils Brünner
Jul 3, 2016·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Warner Alpízar-AlpízarMartin Illemann
Mar 11, 2018·BMC Cancer·Charlotte Aaberg-JessenBjarne W Kristensen
Jul 31, 2013·Virchows Archiv : an International Journal of Pathology·Marius I IliePaul Hofman
Sep 14, 2010·Journal of Neuro-oncology·Jesper KolendaBjarne Winther Kristensen
May 12, 2009·Journal of Neuro-oncology·Charlotte Aaberg-JessenBjarne Winther Kristensen
Dec 20, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Steffen RickeltRichard O Hynes
Feb 6, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kyle J IsaacsonHamidreza Ghandehari