PMID: 6539353Jun 8, 1984Paper

Characterization of antisera to cholecystokinin by use of different cholecystokinin labels

Journal of Immunological Methods
J B Jansen, C B Lamers

Abstract

Antisera raised against synthetic sulphated cholecystokinin (CCK 26-33) (n = 4) and against 30% pure porcine CCK (n = 11) were characterized by the use of different labelled CCK peptides. CCK 39 and CCK 26-33 were coupled to 125I by the chloramine-T method, while CCK 33 was conjugated to 125I-labelled hydroxyphenylpropionic acid-succinimide ester (Bolton-Hunter reagent). Antisera raised against CCK 26-33 bound to 125I-CCK 26-33 only. Of the antisera raised against 30% pure CCK, 2 bound to all 3 labels, 4 to 125I-BH-CCK 33 and 125I-CCK 26-33, 3 to 125I-CCK 39 and 125I-BH-CCK 33, 1 to 125I-CCK 26-33 only and 1 to 125I-BH-CCK 33 only. The antibodies reacting with 125I-CCK 26-33 bound also to 125I-gastrin 1-17. Different CCK labels bound to different binding sites in the same antiserum (antibody heterogeneity). The pattern of reactivity of the antiserum to CCK peptides was dependent on the type of label used. With these different labels, antibodies specific for CCK 39, for CCK 33 and CCK 39, for sulphated forms of CCK, and for all CCK peptides and gastrin could be detected. It is concluded that antisera raised against CCK should be characterized by use of different labelled CCK materials.

References

May 13, 1976·The New England Journal of Medicine·P L RayfordJ C Thompson
Jan 1, 1976·Clinical Endocrinology·V Mutt
Dec 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation·F Stadil, J F Rehfeld
Feb 21, 1967·Biochemical and Biophysical Research Communications·V Mutt, J E Jorpes
Jun 11, 1982·Journal of Immunological Methods·J B Jansen, C B Lamers
Feb 21, 1983·Life Sciences·J B Jansen, C B Lamers
May 1, 1983·Digestive Diseases and Sciences·T M Chang, W Y Chey

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Citations

Sep 3, 1985·Journal of Immunological Methods·P Cantor, J F Rehfeld
Jan 1, 1985·Annals of the New York Academy of Sciences·J B Jansen, C B Lamers
Jan 1, 1992·Scandinavian Journal of Gastroenterology. Supplement·J B JansenC B Lamers
May 20, 1998·Journal of Molecular Recognition : JMR·C J WorkmanE W Voss

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