Characterization of Aplysia carboxypeptidase E

FEBS Letters
S JuvvadiL D Fricker

Abstract

Carboxypeptidase E (CPE) is involved in the biosynthesis of peptide hormones and neurotransmitters. To determine whether a recently reported Aplysia californica cDNA encodes a CPE-like enzyme, this cDNA was expressed in the baculovirus system. The Aplysia CPE is optimal at pH 5.5-6.5 and is inhibited by chelating agents and by the sulfhydryl reagent p-chloromercuriphenyl sulfonate. The effect of divalent cations and active site-directed inhibitors on enzyme activity are generally similar for Aplysia and rat CPE. Western blot analysis using antisera to the N- and C-terminal regions of the Aplysia CPE show that the Aplysia CPE is present in atrial glands and ovotestis. This Aplysia CPE is purified on a p-aminobenzoyl-Arg Sepharose affinity column under conditions that selectively purify rat CPE. Taken together, these results suggest that the previously cloned cDNA represents a CPE-like enzyme that is expressed in Aplysia tissue.

References

Jul 1, 1991·Molecular and Cellular Endocrinology·W W RothB D Noe
Jan 1, 1988·Annual Review of Physiology·L D Fricker
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L D Fricker, S H Snyder
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·S H SettleK C Burtis
Nov 29, 1996·The Journal of Biological Chemistry·L D FrickerL A Devi
Jul 1, 1997·DNA and Cell Biology·X XinL D Fricker

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