Characterization of apoptosis in articular cartilage derived from the knee joints of patients with osteoarthritis.

Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA
Giuseppe MusumeciGiuseppa Martinez

Abstract

the present study was conduced in order to analyse the molecular changes during the apoptotic cascade in knee articular cartilage of patients with OA. articular cartilage specimens were assessed by histology (Haematoxylin and Eosin), histochemistry (Masson's Trichromic and Alcian Blue), immunohistochemistry through TRAIL, DR5 and Caspase-3, TUNEL and Hoechst staining in fresh isolated chondrocytes. histology results demonstrated the structural alterations in the articular knee cartilage with OA, and histochemistry results demonstrated the presence of matrix calcification and a proteoglycans reduction. Immunohistochemistry staining showed that structural alterations, matrix calcification and a proteoglycans reduction coincided with an increase in apoptotic cells when compared to normal cartilage; however, this cellular mechanism of death was demonstrated by TUNEL and Hoechst 33258 staining in fresh isolated chondrocytes. in this study, we demonstrated an apoptosis activation by the extrinsic pathway in OA cartilage. The apoptosis-positive cells might be due to a protection mechanism after sublethal injury, in particular, represented by an increased survival of chondrocytes that are able to participate in the repair process.

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Citations

Nov 9, 2010·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Francisco ForriolVincenzo Denaro
Aug 1, 2013·International Journal of Molecular Sciences·Giuseppe MusumeciAnnelie Martina Weinberg
Apr 4, 2013·Cell and Tissue Banking·Matteo LaganàManuela Teresa Raimondi
Jul 19, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Jun QinLiao-Bin Chen
Sep 1, 2011·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Giuseppe MusumeciCarla Loreto
Feb 9, 2013·Rheumatology International·Melek SezginMehmet Emin Erdal
Dec 25, 2012·Journal of Bone and Mineral Metabolism·Giuseppe MusumeciAnnelie Martina Weinberg
Sep 4, 2015·International Journal of Molecular Sciences·Giuseppe MusumeciAli Mobasheri
Mar 19, 2015·International Journal of Molecular Sciences·Giuseppe MusumeciAli Mobasheri
Jun 1, 2014·Osteoarthritis and Cartilage·R Ruhlen, K Marberry
Mar 19, 2014·Molecular Pharmaceutics·Niels SchlichtingJochen Ringe
Sep 28, 2016·Genetic Testing and Molecular Biomarkers·Gui-Long WangCui-Yun Li
Mar 3, 2018·International Journal of Molecular Sciences·Ju-Ryoung KimHyun Ah Kim
Jul 20, 2017·Journal of Cellular Physiology·Zhen CaoShiwu Dong
May 16, 2014·World Journal of Orthopedics·Giuseppe MusumeciSergio Castorina
Jun 13, 2018·Journal of Cellular Biochemistry·Zhongmeng YangQing Zhao
Jan 1, 2014·F1000Research·Giuseppe MusumeciPaola Castrogiovanni
Feb 11, 2020·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Xiaoliang BaiSidong Yang
Sep 5, 2020·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Paola CastrogiovanniGiuseppe Musumeci
Jan 1, 2015·Arthritis Research & Therapy·Naga Padmini KaramcheduGregory D Jay
Jan 1, 2014·F1000Research·Giuseppe MusumeciPaola Castrogiovanni
Apr 29, 2020·Drug Design, Development and Therapy·Hui GaoPing Li
Nov 8, 2020·Annals of the Rheumatic Diseases·Wenhui HuShiwu Dong

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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