Abstract
Liver cancer is a fatal disease with limited therapy options. The recombinant adenovirus expressing tumor-suppressor gene of PTEN (Ad5-PTEN) showed effective antitumor activity against liver cancer. However, its disadvantages produced great limitation on its application, especially its nonspecific and toxicity to normal cells and tissues. The epithelial cell adhesion molecule (EpCAM) is over-expressed in some liver cancer cells and an RNA aptamer EpDT3 could specially target to EpCAM-positive cells. Based on this founding, we aimed to design a kind of gene delivery system of EpDT3-mediated Ad5-PTEN (EpDT3-PEG-Ad5-PTEN, EPAP) in which polyethylene glycol was used to be a linker to conjugate EpDT3 with Ad5-PTEN. This strategy may overcome the disadvantages of naked Ad5-PTEN and enhance the antitumor effect on liver cancer. The SDS-PAGE electrophoresis, TBE-PAGE electrophoresis and fluorescence detection were conducted to confirm the successful preparation of EPAP. Compared with the naked Ad5-PTEN, EPAP showed significant anti-proliferative and anti-migratory activities against HepG2 cells. EPAP also showed selective and precise target ability to EpCAM-positive HepG2 cellsin vivo. Therefore, EPAP may be further explored as a novel...Continue Reading
References
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D PapahadjopoulosC Redemann
Nov 1, 1996·Journal of Virology·N MitterederB C Trapnell
Mar 28, 1997·Science·J LiR Parsons
Jul 1, 1997·Nature Medicine·C Nyberg-HoffmanE Aguilar-Cordova
Jan 24, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Josep M Llovet, Jordi Bruix
Sep 12, 2008·Nature Reviews. Cancer·Jane E Visvader, Geoffrey J Lindeman
Dec 9, 2009·Molecular Pharmaceutics·Zhirong ZhongXun Sun
Jan 5, 2011·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·C P HsuY C Chung
Feb 2, 2011·Cancer Science·Sarah ShigdarWei Duan
Jun 24, 2011·International Journal of Nanomedicine·Zhirong ZhongXun Sun
Oct 12, 2012·International Journal of Nanomedicine·Zhongbing LiuZhirong Zhong
Nov 28, 2014·Advances in Experimental Medicine and Biology·Colin T Shearn, Dennis R Petersen
Jan 20, 2015·Oncogene·X ZhaoX W Wang
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Apr 7, 2015·Gastrointestinal Cancer : Targets and Therapy·Adam B SendorAutumn J McRee
Aug 1, 2015·BoneKEy Reports·Kiyomi YamazakiStephen L-K Yen
Nov 17, 2015·International Journal of Pharmaceutics·Dan XiongZhirong Zhong
Oct 25, 2016·Cancer Letters·Pei JingZhirong Zhong
May 20, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shuangli XiaoZhirong Zhong