PMID: 9178499May 1, 1997Paper

Characterization of arenaviruses using a family-specific primer set for RT-PCR amplification and RFLP analysis. Its potential use for detection of uncharacterized arenaviruses

Virus Research
M LozanoV Romanowski

Abstract

Arenaviruses are enveloped viruses with a genome composed of two ssRNA species, designated L and S. The arenaviruses were divided in two major groups (Old World and New World), based on serological properties and genetic data, as well as geographic distribution. A sequence alignment analysis of all reported arenavirus S RNAs yielded 17 conserved regions in addition to a reported conserved region at the end of both RNAs. The consensus sequences of these regions were used to design generalized primers suitable for RT-PCR amplification of a set of overlapping nucleotide sequence fragments comprising the complete S RNA of any arenavirus. A restriction analysis (RFLP) was designed to rapidly typify the amplified fragments. This RT-PCR-RFLP approach was tested with Old World (LCM) and New World (Junin and Tacaribe) arenaviruses. Furthermore, using this procedure the whole S RNA of a novel arenavirus isolate obtained from a rodent trapped in central Argentina, was amplified and characterized. Partial nucleotide sequence data were used for phylogenetic analyses that showed the relationships between this arenavirus and the rest of the members of the family. This relatively simple methodology will be useful both in basic studies and epid...Continue Reading

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Citations

Mar 10, 2006·Virus Genes·Sandra Elizabeth GoñiMario Enrique Lozano
Dec 17, 2003·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jonathan P Clewley
Mar 22, 2002·Journal of Virological Methods·María A MoralesMarta S Sabattini
Jul 22, 1998·Journal of Virological Methods·G Kuno
Mar 5, 2003·Antiviral Research·Rémi N Charrel, Xavier de Lamballerie
Sep 1, 2007·Vector Borne and Zoonotic Diseases·James N MillsPeter B Jahrling
Jul 11, 2000·Journal of Virology·M D BowenS T Nichol
Nov 23, 2012·Emerging Infectious Diseases·Allan KessellIna Smith
Dec 4, 2012·Viruses·Igor S Lukashevich
Jan 25, 2013·Viruses·Juan C Zapata, Maria S Salvato
Sep 15, 2009·Veterinary Microbiology·R N Charrel, X de Lamballerie
Oct 19, 2004·Critical Reviews in Clinical Laboratory Sciences·Stephan Günther, Oliver Lenz
Dec 22, 2012·Expert Review of Vaccines·Igor S Lukashevich
Dec 8, 2019·EcoHealth·Julius NzizaKirsten Gilardi
Oct 10, 2006·Pediatric Clinics of North America·Jiang FanKelly J Henrickson

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