Characterization of Argonaute cDNA from Penaeus monodon and implication of its role in RNA interference

Biochemical and Biophysical Research Communications
Manasave DechklarSakol Panyim

Abstract

RNA interference (RNAi) has recently become a promising strategy for therapeutic of several viral diseases including those in the black tiger shrimp Penaeus monodon. However, the protein components that play role in RNAi in P. monodon have not yet been identified. Here, we report the cloning and functional characterization of a cDNA encoding Argonaute, a principal constituent of RNAi pathway in P. monodon. P. monodon's Argonaute (Pem-AGO) exhibited the two signature domains, PAZ and PIWI. Substantial level of Pem-ago expression could be suppressed by double-stranded RNA (dsRNA) that targeted PAZ coding sequence in shrimp primary culture of Oka cells. The Pem-ago depleted cells showed impaired RNAi as the expression of an endogenous gene was rescued from the dsRNA-mediated silencing in these cells. Our results imply that Pem-ago is required for effective RNAi in P. monodon and thus identify the first protein constituent of RNAi machinery in penaeid shrimp.

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Citations

Apr 17, 2010·Marine Biotechnology·Ikuo HironoTakashi Aoki
Jan 22, 2013·Fish & Shellfish Immunology·Amnat PhetrungnaphaChalermporn Ongvarrasopone
Jan 2, 2013·Fish & Shellfish Immunology·Paula C LimaMathew Cook
Jun 27, 2012·Fish & Shellfish Immunology·Yannick Labreuche, Gregory W Warr
Mar 21, 2012·Journal of Invertebrate Pathology·Lyric C BartholomayD L Harris
Jul 13, 2011·Fish & Shellfish Immunology·Amnat PhetrungnaphaChalermporn Ongvarrasopone
Jun 11, 2011·Fish & Shellfish Immunology·Lingwei RuanXiumin Yan
Aug 29, 2009·Journal of Virological Methods·Vanvimon SaksmerpromeBoonsirm Withyachumnarnkul
Feb 19, 2009·Fish & Shellfish Immunology·Haipeng LiuPikul Jiravanichpaisal
Jan 6, 2012·The Journal of General Virology·J Dustin LoyLyric C Bartholomay
Jul 5, 2013·Fish & Shellfish Immunology·Amnat PhetrungnaphaChalermporn Ongvarrasopone
Dec 5, 2012·Fish & Shellfish Immunology·Lishi YangShigui Jiang
May 3, 2014·Biology of Reproduction·Siuming Francis ChanCheng Bo Sun

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