Characterization of aromatase binding agents from the dichloromethane extract of Corydalis yanhusuo using ultrafiltration and liquid chromatography tandem mass spectrometry.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Jing ShiFang Sun

Abstract

Aromatase represents an important target for the treatment of hormone-dependent breast cancer. In the present study, nine alkaloids from the dichloromethane extract of Corydalis yanhusuo were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) and tested for their aromatase binding activities using an ultrafiltration LC-MS method by investigating the differences of peak areas of compounds before and after incubations with aromatase. It was demonstrated that the quaternary protoberberine alkaloids and the tertiary protoberberine alkaloids exhibited potent aromatase binding activities. The quaternary ammonium group and the methyl group at C-13 position of tertiary protoberberine alkaloids might be necessary for the activity. The findings should provide guidance for the discovery of potential aromatase inhibitors from natural products.

References

Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C T EvansC R Mendelson
Oct 13, 1994·European Journal of Pharmacology·P C NgG Stone
Dec 20, 1993·Cancer Letters·N KadohamaY Osawa
Feb 3, 1999·Cancer Letters·J C Le BailG Habrioux
Aug 26, 2000·Analytical Chemistry·D NikolicR B van Breemen
May 23, 2001·Journal of Agricultural and Food Chemistry·J LiuJ L Bolton
Oct 27, 2001·Journal of Natural Products·D LeeA D Kinghorn
Sep 6, 2003·Steroids·Richard J Santen
Jan 8, 2004·British Journal of Cancer·Z-W Wong, M J Ellis
Apr 7, 2004·Journal of the American Geriatrics Society·Amit Arora, Jane F Potter
Aug 21, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·J Thomas SandersonMartin van den Berg
Sep 11, 2004·The Journal of Biological Chemistry·Shiuan ChenYate-Ching Yuan
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Yongkai SunRichard B van Breemen
Apr 9, 2005·Endocrine Reviews·Robert W BrueggemeierEdgar S Diaz-Cruz
Apr 14, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yun WangLai K Leung
Jul 1, 2006·Cancer Investigation·Michael Gnant
Sep 7, 2006·Endocrine-related Cancer·Anne Kendall, Mitch Dowsett
Oct 6, 2006·Expert Opinion on Pharmacotherapy·Robert W Brueggemeier
Nov 18, 2006·Phytochemistry·Lenka GrycováRadek Marek
Aug 21, 2007·The Journal of Organic Chemistry·Yasuhiro WadaKazuhiko Orito
Oct 3, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhong-Ze MaDavid Y W Lee

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Citations

Jan 20, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhiying DouLiu Cao
Apr 1, 2016·Journal of Food and Drug Analysis·Hsin-Yi Hung, Tian-Shung Wu

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