Characterization of Ascentis RP-Amide column: Lipophilicity measurement and linear solvation energy relationships

Journal of Chromatography. a
Deborah Benhaim, Eli Grushka

Abstract

This study investigates lipophilicity determination by chromatographic measurements using the polar embedded Ascentis RP-Amide stationary phase. As a new generation of amide-functionalized silica stationary phase, the Ascentis RP-Amide column is evaluated as a possible substitution to the n-octanol/water partitioning system for lipophilicity measurements. For this evaluation, extrapolated retention factors, log k'w, of a set of diverse compounds were determined using different methanol contents in the mobile phase. The use of n-octanol enriched mobile phase enhances the relationship between the slope (S) of the extrapolation lines and the extrapolated log k'w (the intercept of the extrapolation),as well as the correlation between log P values and the extrapolated log k'w (1:1 correlation, r2 = 0.966).In addition, the use of isocratic retention factors, at 40% methanol in the mobile phase, provides a rapid tool for lipophilicity determination. The intermolecular interactions that contribute to the retention process in the Ascentis RP-Amide phase are characterized using the solvation parameter model of Abraham.The LSER system constants for the column are very similar to the LSER constants of the n-octanol/water extraction system....Continue Reading

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Citations

Aug 9, 2011·Journal of Chromatography. a·Juan M PallicerElisabeth Bosch
Nov 28, 2012·Journal of Separation Science·Imran AliKamlesh K Dutta
Mar 12, 2016·Expert Opinion on Drug Discovery·Fotios TsopelasAnna Tsantili-Kakoulidou
Oct 12, 2013·Journal of Separation Science·Silvia EcheverríaEva Pocurull
Jun 21, 2016·Journal of Chromatography. a·Colin F Poole, Nicole Lenca
Aug 3, 2020·Journal of Chromatography. a·Colin F Poole, Sanka N Atapattu

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