PMID: 11333315May 3, 2001Paper

Characterization of ascorbate peroxidases from unicellular red alga Galdieria partita

Plant & Cell Physiology
S SanoA Yokota

Abstract

Galdieria partita, a unicellular red alga isolated from acidic hot springs and tolerant to sulfur dioxide, has at least two ascorbate peroxidase (APX) isozymes. This was the first report to demonstrate that two isozymes of APX are found in algal cells. Two isozymes were separated from each other at the hydrophobic chromatography step of purification and named APX-A and APX-B after the elution order in the chromatography. APX-B accounted for 85% of the total activity. Both isozymes were purified. APXs from Galdieria were monomers whose molecular weights were about 28,000, similar to stromal APX of higher plants. APX-A cross-reacted with monoclonal antibody raised against APX of Euglena gracilis in immunoblotting, but APX-B did not, although the antibody can recognize all other APXs tested. The amino-terminal sequences of APX-A and -B from Galdieria had some homology with each other but little homology with those from other sources. Their Km values for ascorbate and hydrogen peroxide were comparable with those of APX from higher plants. Unlike the green algal enzymes, the donor specificities of Galdieria APXs were as high as those of plant chloroplastic APX. On the contrary, these APXs reduced tertiary-butyl hydroperoxide as an e...Continue Reading

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Citations

Dec 16, 2004·Journal of Molecular Evolution·Felipe Karam TeixeiraMárcia Margis-Pinheiro
Sep 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Shane R WilkinsonJohn M Kelly
Oct 31, 2012·Journal of Experimental Botany·Noé GestRebecca Stevens
Feb 16, 2013·Genetics and Molecular Biology·Andréia CaverzanMárcia Margis-Pinheiro
Sep 22, 2020·AMB Express·Dora Allegra CarboneMichael Melkonian
Apr 30, 2020·American Journal of Physiology. Cell Physiology·Amanda M EccardtJonathan S Fisher
Jan 30, 2020·Toxicology Reports·Maryam RezayianHassan Ebrahimzadeh
May 6, 2019·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mark H ManninoJonathan S Fisher

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