Characterization of Asymptomatic Bacteriuria Escherichia coli Isolates in Search of Alternative Strains for Efficient Bacterial Interference against Uropathogens

Frontiers in Microbiology
Christoph StorkUlrich Dobrindt

Abstract

Asymptomatic bacterial colonization of the urinary bladder (asymptomatic bacteriuria, ABU) can prevent bladder colonization by uropathogens and thus symptomatic urinary tract infection (UTI). Deliberate bladder colonization with Escherichia coli ABU isolate 83972 has been shown to outcompete uropathogens and prevent symptomatic UTI by bacterial interference. Many ABU isolates evolved from uropathogenic ancestors and, although attenuated, may still be able to express virulence-associated factors. Our aim was to screen for efficient and safe candidate strains that could be used as alternatives to E. coli 83972 for preventive and therapeutic bladder colonization. To identify ABU E. coli strains with minimal virulence potential but maximal interference efficiency, we compared nine ABU isolates from diabetic patients regarding their virulence- and fitness-associated phenotypes in vitro, their virulence in a murine model of sepsis and their genome content. We identified strains in competitive growth experiments, which successfully interfere with colonization of ABU isolate 83972 or uropathogenic E. coli strain 536. Six isolates were able to outcompete E. coli 83972 and two of them also outcompeted UPEC 536 during growth in urine. Sup...Continue Reading

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Citations

Jan 1, 2019·Current Bladder Dysfunction Reports·A Lenore Ackerman, Toby C Chai
Feb 20, 2020·Nature Reviews. Microbiology·Roger D Klein, Scott J Hultgren
Feb 16, 2019·Journal of Veterinary Internal Medicine·Anaïs LamoureuxChristelle Maurey
Feb 6, 2021·Mucosal Immunology·Bernadette Jones-FreemanMalcolm R Starkey

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Datasets Mentioned

BETA
SRR6224591

Methods Mentioned

BETA
urine sample collection

Software Mentioned

FastQC
po2group
Prokka
SerotypeFinder
PlasmidFinder
R
ResFinder
Proteinortho
Roary
cds

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