Characterization of Atlantic halibut (Hippoglossus hippoglossus) Mx protein expression

Developmental and Comparative Immunology
Veronica Bergan, Børre Robertsen

Abstract

Mx proteins are antiviral GTPases that are induced by type I interferons in vertebrates. An Atlantic halibut Mx cDNA (HHMx) was recently cloned. In this work, a polyclonal antiserum against HHMx protein was generated that detected a 71 kDa protein in the nuclei of Chinook salmon embryo cells transfected with the HHMx cDNA. Mx protein expression in organs of halibut was studied by immunoblot analysis after injection with the double-stranded RNA poly I:C or infectious pancreatic necrosis virus. Poly I:C stimulated increased Mx protein expression in liver, kidney, heart, spleen, gills and intestine. The Mx protein level in liver reached a maximum after 3 days and remained elevated for 14 days after treatment. IPNV infection resulted in increased Mx protein in liver from 4 to at least 35 days. Immunocytochemical detection of Mx proteins in blood smears from poly I:C treated halibut indicated that a cytoplasmic Mx form might exist in this species. Detection of Mx proteins in blood leukocytes could thus work as an early non-lethal test for viral infections.

References

Jan 1, 1990·Advances in Virus Research·P Staeheli
Dec 1, 1990·Journal of Virology·E MeierH Arnheiter
Feb 1, 1995·Experimental Eye Research·M E AbramsC R Brandt
Jun 1, 1993·Virology·E A GarberL H van der Ploeg
Aug 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·G D Trobridge, J A Leong
Dec 16, 1997·Developmental and Comparative Immunology·B RobertsenJ A Leong
Apr 30, 1998·Journal of Virological Methods·V ChieuxP Wattré
Jun 25, 1998·Revue Scientifique Et Technique·O HallerG Kochs
Mar 29, 2000·Developmental and Comparative Immunology·J Y LeeT Aoki
Aug 24, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·V Jensen, B Robertsen
Sep 20, 2000·Fish & Shellfish Immunology·R NygaardB Robertsen
Oct 5, 2001·Clinical Microbiology Reviews·C E Samuel
Feb 16, 2002·The Journal of Biological Chemistry·Georg KochsOtto Haller
Jan 15, 2003·Journal of Virology·Stephen M AltmannCarol H Kim
Sep 13, 2003·Developmental and Comparative Immunology·Scott LongV G Chinchar
Oct 31, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Børre RobertsenArtur Albuquerque
Dec 17, 2003·Fish & Shellfish Immunology·C TafallaB Novoa
Dec 31, 2003·Developmental and Comparative Immunology·Stephen M AltmannCarol H Kim
Dec 31, 2003·Virology·Christopher Marlowe A CaipangTakashi Aoki
May 5, 2004·Fish & Shellfish Immunology·Karen P Plant, Ronald L Thune
Jul 16, 2004·Journal of Virology·Rannveig LarsenBørre Robertsen

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Citations

Jun 9, 2005·Fish & Shellfish Immunology·Børre Robertsen
Oct 23, 2012·Fish & Shellfish Immunology·Bjørn Olav KvammeØystein Evensen
Dec 23, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Christopher Marlowe A CaipangTakashi Aoki
Aug 30, 2008·Fish & Shellfish Immunology·Elisa RandelliGiuseppe Scapigliati
Jan 29, 2008·Developmental and Comparative Immunology·Young-Mao ChenTzong-Yueh Chen
Dec 15, 2007·Fish & Shellfish Immunology·M Alejandra Fernández-TrujilloJulia Béjar
Nov 6, 2007·Fish & Shellfish Immunology·Shona K Whyte
Jun 13, 2006·Fish & Shellfish Immunology·M Alejandra Fernández-TrujilloJulia Béjar
Nov 1, 2016·Genome Biology and Evolution·Monica H SolbakkenSissel Jentoft
Jan 8, 2013·Journal of Aquatic Animal Health·H OgutR Parlak

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