Characterization of Atropa belladonna L. compounds by capillary electrophoresis-electrospray ionization-time of flight-mass spectrometry and capillary electrophoresis-electrospray ionization-ion trap-mass spectrometry

Electrophoresis
David Arráez-RománAlberto Fernández-Gutiérrez

Abstract

This short communication describes the characterization of seven tropane alkaloid compounds in Atropa belladonna L. Thus a rapid and easy CE-electrospray interface (ESI)-TOF-MS procedure is developed to analyze these compounds in a pharmaceutical preparations of A. belladonna L. leaf extract. Optimum electrophoretic separation is obtained using an alkaline solution of 60 mM ammonium acetate at pH 8.5 containing 5% isopropanol. Under the optimum CE-ESI-TOF-MS conditions several important compounds such as tropine, belladonnine, norhyoscyamine, apoatropine, hyoscyamine, 6beta-hydroxyhyoscyamine, and scopolamine have been simultaneously identified from A. belladonna L. CE-ESI-IT-MS has been used to discriminate the putative presence of littorine. The sensitivity, together with mass accuracy and true isotopic pattern of the TOF-MS, allowed the identification of a broad series of tropane alkaloid compounds present in pharmaceutical preparations of A. belladonna L. leaf extract.

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Citations

Apr 11, 2013·Journal of Chromatography. a·Stephanie KrügerGertrud E Morlock
Dec 25, 2010·Journal of Pharmaceutical and Biomedical Analysis·Roberto Gotti
Apr 13, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elke Aehle, Birgit Dräger
Jun 12, 2009·Electrophoresis·Christian W Klampfl
Dec 8, 2011·Electrophoresis·Heide R RabanesJoselito P Quirino
Dec 6, 2011·Electrophoresis·Xiao-jia ChenShao-Ping Li
Aug 14, 2012·Proteomics·Shuai Sherry ZhaoDavid D Y Chen
Nov 6, 2013·Science & Justice : Journal of the Forensic Science Society·Jorge SáizCarmen García-Ruiz
May 15, 2009·Electrophoresis·Aline StaubJean-Luc Veuthey

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