Characterization of AtSEC12 and AtSAR1. Proteins likely involved in endoplasmic reticulum and Golgi transport

Plant Physiology
M Bar-Peled, N V Raikhel

Abstract

Transport of cargo proteins from the endoplasmic reticulum (ER) to the cis-Golgi network is mediated by protein-coated vesicles. The coat, called COPII coat, consists of proteins that are recruited from the cytosol and interact with integral membrane proteins of the ER. In yeast, both cytosolic proteins (Sec13/31, Sec23/24, and Sar1) and ER-associated proteins (Sec12 and others) have been purified and characterized and it has been possible to demonstrate transport vesicle formation in vitro. Arabidopsis thaliana homologs of Sar1 and Sec12 have recently been identified, but little is known about the properties of the proteins or their subcellular distribution. Here we demonstrate that AtSAR1, a 22-kD protein that binds GTP, and AtSEC12, a 43-kD GTP-exchange protein, are both associated with the ER. However, about one-half of the cellular AtSAR1 is present in the cytosol. When AtSAR1 is overexpressed in transgenic plants, the additional protein is also cytosolic. When tissue-culture cells are cold-shocked (12 h at 8 degrees C), AtSAR1 levels appeared to decline and a larger proportion of the total protein was found in the cytosol. Given the known function of AtSAR1 in yeast, we propose that the amount of ER-associated AtSAR1 is a...Continue Reading

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Citations

Jan 19, 2005·Planta·Sivakumar PattathilMaor Bar-Peled
Apr 5, 2001·Trends in Plant Science·A Nebenführ, L A Staehelin
Jul 25, 2003·Trends in Plant Science·Gloria K MudayAngus S Murphy
Oct 24, 2002·Current Opinion in Plant Biology·Andreas Nebenführ
Mar 6, 1999·Current Opinion in Plant Biology·K Matsuoka, S Y Bednarek
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·B LeymanM R Blatt
Feb 18, 2003·Molecular Biology of the Cell·Enrique RojoNatasha V Raikhel
Oct 29, 2009·Journal of Experimental Botany·Anne OsterriederChris Hawes
Jun 18, 2002·Plant Physiology·Haiyan ZhengNatasha V Raikhel
Jan 20, 2004·Plant Physiology·Daniel GriesenHan Asard
Aug 6, 2008·Plant Physiology·Erik NielsenTakashi Ueda
May 25, 2004·The Plant Cell·Mary L PreussErik Nielsen
Apr 24, 1999·The Plant Cell·A A Sanderfoot, N V Raikhel
Nov 23, 2000·The Plant Cell·P PimplD G Robinson
Sep 11, 2001·The Plant Cell·B A PhillipsonJ Denecke
Feb 22, 2005·The New Phytologist·Chris Hawes
Nov 26, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·David J McKinnonGordon R Gray
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Gerd Jurgens
Sep 10, 2013·International Journal of Molecular Sciences·Birsen Cevher-Keskin
Jan 1, 2008·The Arabidopsis Book·Diane C BasshamAnton A Sanderfoot
Jun 30, 2000·Biochemical and Biophysical Research Communications·I ContrerasF Aniento
Dec 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bryon S DonohoeL Andrew Staehelin

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