Characterization of Avt1p as a vacuolar proton/amino acid antiporter in Saccharomyces cerevisiae

Bioscience, Biotechnology, and Biochemistry
Junichi ToneYoshimi Kakinuma

Abstract

Several genes for vacuolar amino acid transport were reported in Saccharomyces cerevisiae, but have not well been investigated. We characterized AVT1, a member of the AVT vacuolar transporter family, which is reported to be involved in lifespan of yeast. ATP-dependent uptake of isoleucine and histidine by the vacuolar vesicles of an AVT exporter mutant was lost by introducing avt1∆ mutation. Uptake activity was inhibited by the V-ATPase inhibitor: concanamycin A and a protonophore. Isoleucine uptake was inhibited by various neutral amino acids and histidine, but not by γ-aminobutyric acid, glutamate, and aspartate. V-ATPase-dependent acidification of the vesicles was declined by the addition of isoleucine or histidine, depending upon Avt1p. Taken together with the data of the amino acid contents of vacuolar fractions in cells, the results suggested that Avt1p is a proton/amino acid antiporter important for vacuolar compartmentalization of various amino acids.

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Citations

Sep 2, 2015·Bioscience, Biotechnology, and Biochemistry·Miyuki Kawano-KawadaYoshimi Kakinuma
Mar 2, 2016·Bioscience, Biotechnology, and Biochemistry·Kunio ManabeYoshimi Kakinuma
Jun 18, 2015·Bioscience, Biotechnology, and Biochemistry·Siriporn LunpromKoichi Akiyama
Oct 3, 2018·Biological & Pharmaceutical Bulletin·Miyuki Kawano-KawadaTakayuki Sekito
May 30, 2019·Molecular Biology of the Cell·Jenna M GoodrumAdam L Hughes
Oct 18, 2019·Microbiology and Molecular Biology Reviews : MMBR·Frans BianchiBert Poolman
Feb 25, 2021·Bioscience, Biotechnology, and Biochemistry·Akane SatoTakayuki Sekito
Mar 12, 2021·Bioscience, Biotechnology, and Biochemistry·Miyuki Kawano-KawadaTakayuki Sekito
Mar 11, 2021·PLoS Genetics·Raffaele NicastroClaudio De Virgilio
Nov 16, 2020·Biochimica Et Biophysica Acta. Biomembranes·Miyuki Kawano-KawadaTakayuki Sekito

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Methods Mentioned

BETA
fluorescence microscopy
PCR
Fluorescence
amino acid

Software Mentioned

TMpred
MetaMorph
TMHMM
TreeView
ClustalW
DAS

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