Characterization of axon guidance cue sensitivity of human embryonic stem cell-derived dopaminergic neurons

Molecular and Cellular Neurosciences
Branden CordMary A Hynes

Abstract

Dopaminergic neurons derived from human embryonic stem cells will be useful in future transplantation studies of Parkinson's disease patients. As newly generated neurons must integrate and reconnect with host cells, the ability of hESC-derived neurons to respond to axon guidance cues will be critical. Both Netrin-1 and Slit-2 guide rodent embryonic dopaminergic (DA) neurons in vitro and in vivo, but very little is known about the response of hESC-derived DA neurons to any axonal guidance cues. Here we examined the ability of Netrin-1 and Slit-2 to affect human ESC DA axons in vitro. hESC DA neurons mature over time in culture with the developmental profile of DA neurons in vivo, including expression of the DA neuron markers FoxA2, En-1 and Nurr-1, and receptors for both Netrin and Slit. hESC DA neurons respond to exogenous Netrin-1 and Slit-2, showing an increased responsiveness to Netrin-1 as the neurons mature in culture. These responses were maintained in the presence of pro-inflammatory cytokines that might be encountered in the diseased brain. These studies are the first to evaluate and confirm that suitably matured human ES-derived DA neurons can respond appropriately to axon guidance cues.

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Citations

Jan 6, 2012·Annals of Biomedical Engineering·Aleesha M McCormick, Nic D Leipzig
Jan 10, 2013·Experimental Neurology·Fahad AliRoger A Barker
Apr 10, 2015·Neuron·Julius A Steinbeck, Lorenz Studer
Oct 30, 2013·Journal of Neurochemistry·Timothy M Gomez, Paul C Letourneau
Jan 21, 2018·The Journal of Cell Biology·Fabio L UrbinaStephanie L Gupton
Dec 5, 2019·Proceedings of the National Academy of Sciences of the United States of America·Janos FuzikTibor Harkany

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