PMID: 9171403Jun 1, 1997Paper

Characterization of Azorhizobium caulinodans glnB and glnA genes: involvement of the P(II) protein in symbiotic nitrogen fixation

Journal of Bacteriology
N Michel-ReydelletP A Kaminski

Abstract

The nucleotide sequence and transcriptional organization of Azorhizobium caulinodans ORS571 glnA, the structural gene for glutamine synthetase (GS), and glnB, the structural gene for the P(II) protein, have been determined. glnB and glnA are organized as a single operon transcribed from the same start site, under conditions of both nitrogen limitation and nitrogen excess. This start site may be used by two different promoters since the expression of a glnB-lacZ fusion was high in the presence of ammonia and enhanced under conditions of nitrogen limitation in the wild-type strain. The increase was not observed in rpoN or ntrC mutants. In addition, this fusion was overexpressed under both growth conditions, in the glnB mutant strain, suggesting that P(II) negatively regulates its own expression. A DNA motif, similar to a sigma54-dependent promoter consensus, was found in the 5' nontranscribed region. Thus, the glnBA operon seems to be transcribed from a sigma54-dependent promoter that operates under conditions of nitrogen limitation and from another uncharacterized promoter in the presence of ammonia. Both glnB and glnBA mutant strains derepress their nitrogenase in the free-living state, but only the glnBA mutant, auxotrophic fo...Continue Reading

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Citations

Oct 28, 1999·Nucleic Acids Research·H BarriosE Morett
Aug 7, 2012·FEMS Microbiology Reviews·Luciano F HuergoMike Merrick
May 19, 2001·Canadian Journal of Microbiology·E M BenelliF O Pedrosa
Jun 12, 2003·FEMS Microbiology Letters·Luciano F HuergoLeda S Chubatsu
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·T ArcondéguyM Merrick
Apr 10, 1999·Journal of Bacteriology·N Michel-Reydellet, P A Kaminski

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