Characterization of Bacillus stearothermophilus infA and of its product IF1

Stavroula KapralouR Spurio


Bacillus stearothermophilus infA encoding translation initiation factor IF1 was cloned and expressed in Escherichia coli and its transcript and protein product characterized. Although the functional properties of B. stearothermophilus and E. coli IF1, compared in several translational tests in the presence of both homologous and heterologous components, are not entirely identical, the two proteins are interchangeable in an in vitro translational system programmed with a natural mRNA. The availability of purified B. stearothermophilus IF1 now allows us to analyze the translation initiation pathway using efficient in vitro tests based entirely on purified components derived from this thermophilic Gram-positive bacterium.


Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·N C Kyrpides, C R Woese
Oct 9, 2002·Current Protein & Peptide Science·R Boelens, C O Gualerzi
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·C O GualerziC L Pon
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenHans Uffe Sperling-Petersen
Aug 30, 2006·Biochimie·Victor V CroitoruLeif A Isaksson
Jun 24, 2008·Molecular Cell·Pohl MilonMarina V Rodnina


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May 31, 2019·International Journal of Systematic and Evolutionary Microbiology·Ekaterina M SemenovaTamara N Nazina

Related Concepts

Geobacillus stearothermophilus
Bacterial Proteins
Alkalescens-Dispar Group
Peptide Chain Initiation, Translational
RNA, Bacterial
Poly(A) Tail
Northern Blot
Western Blot
Eukaryotic Initiation Factor-1
Homologous Sequences, Amino Acid

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