Characterization of bacterial beta-carotene 3,3'-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli

Applied Microbiology and Biotechnology
Seon-Kang ChoiNorihiko Misawa

Abstract

beta-Carotene hydroxylase (CrtZ) is one of rate-limiting enzymes for astaxanthin production. A complementation analysis was conducted using Escherichia coli transformants to compare the catalytic efficiency of bacterial CrtZ from Brevundimonas sp. SD212, Paracoccus sp. PC1 (formerly known as Alcaligenes sp. PC-1), Paracoccus sp. N81106 (Agrobacterium aurantiacum), Pantoea ananatis (Erwinia uredovora 20D3), marine bacterium P99-3, and P450 monooxygenase (CYP175A1) from Thermus thermophilus HB27. Each crtZ or CYP175A1 gene was expressed in E. coli transformants synthesizing canthaxanthin and beta-carotene due to the respective presence of plasmids pAC-Cantha and pACCAR16DeltacrtX. The carotenoids that accumulated in the resulting recombinant E. coli cells were examined by chromatographic and spectroscopic analyses. E. coli carrying Brevundimonas sp. SD212 crtZ showed the highest astaxanthin production efficiency among the transformants examined, while there was no significant difference in the catalytic efficiency for conversion from beta-carotene to zeaxanthin. Recombinant E. coli expressing the CYP175A1 gene, in addition to the genes for canthaxanthin synthesis, surprisingly accumulated adonirubin (phoenicoxanthin) as the main ...Continue Reading

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Citations

Apr 7, 2007·Applied Microbiology and Biotechnology·Seon-Kang ChoiNorihiko Misawa
Nov 3, 2010·Applied Microbiology and Biotechnology·Holger BeuttlerVlada B Urlacher
Jan 6, 2007·Journal of Industrial Microbiology & Biotechnology·Rick W YeEdward S Miller
May 24, 2012·Journal of Natural Products·Mark A ScaifeRoberto E Armenta
Feb 11, 2009·Journal of Experimental Botany·Masaki FujisawaNorihiko Misawa
Dec 24, 2014·Journal of Industrial Microbiology & Biotechnology·Xi-Ran LiJian-Zhong Liu
Nov 26, 2013·Applied Microbiology and Biotechnology·Sabine A E HeiderVolker F Wendisch
May 23, 2008·The Plant Journal : for Cell and Molecular Biology·Tomohisa HasunumaChikahiro Miyake
Nov 10, 2010·Natural Product Reports·Haoran ZhangBlaine A Pfeifer
Jul 13, 2019·Journal of Industrial Microbiology & Biotechnology·Yuanqing WuXueming Zhao
Mar 28, 2020·The Journal of General and Applied Microbiology·Naoya ShimadaMasahiko Ikeuchi
Aug 17, 2016·Applied Microbiology and Biotechnology·Hendrik PollmannGerhard Sandmann
Sep 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hubert AntolakDorota Kręgiel
Dec 15, 2018·Physiologia Plantarum·Barbara MeninAnna Paola Casazza
Dec 30, 2020·Progress in Lipid Research·Xia WanQian-Chun Deng

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