Characterization of Bacterial Communities in Selected Smokeless Tobacco Products Using 16S rDNA Analysis

PloS One
Robert E TyxClifford H Watson

Abstract

The bacterial communities present in smokeless tobacco (ST) products have not previously reported. In this study, we used Next Generation Sequencing to study the bacteria present in U.S.-made dry snuff, moist snuff and Sudanese toombak. Sample diversity and taxonomic abundances were investigated in these products. A total of 33 bacterial families from four phyla, Actinobacteria, Firmicutes, Proteobacteria and Bacteroidetes, were identified. U.S.-produced dry snuff products contained a diverse distribution of all four phyla. Moist snuff products were dominated by Firmicutes. Toombak samples contained mainly Actinobacteria and Firmicutes (Aerococcaceae, Enterococcaceae, and Staphylococcaceae). The program PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) was used to impute the prevalence of genes encoding selected bacterial toxins, antibiotic resistance genes and other pro-inflammatory molecules. PICRUSt also predicted the presence of specific nitrate reductase genes, whose products can contribute to the formation of carcinogenic nitrosamines. Characterization of microbial community abundances and their associated genomes gives us an indication of the presence or absence of pathways of int...Continue Reading

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Citations

Jul 10, 2018·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Jessica L ElfJonathan E Golub
Jan 16, 2020·PloS One·Tameka S LawlerClifford H Watson
Jun 18, 2020·Tobacco Prevention & Cessation·Rashmi MehraShivam Kapoor
Apr 23, 2017·Applied Microbiology and Biotechnology·Eoghan M SmythAmy R Sapkota
Aug 25, 2018·World Journal of Microbiology & Biotechnology·Sayak MitraBiswanath Mahanty
Nov 12, 2019·Frontiers in Microbiology·Eoghan M SmythEmmanuel F Mongodin
Nov 13, 2020·Applied Microbiology and Biotechnology·A J RiveraC H Watson
Mar 12, 2021·Applied Microbiology and Biotechnology·Suhana ChattopadhyayAmy R Sapkota
Jun 7, 2021·Anaerobe·Mohammad SajidMausumi Bharadwaj
Jun 11, 2021·Applied Microbiology and Biotechnology·A J Rivera, R E Tyx
Aug 1, 2021·Applied Microbiology and Biotechnology·Akanksha Vishwakarma, Digvijay Verma
Nov 19, 2021·Applied Microbiology and Biotechnology·Jianbin YeZhan Zhang

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Datasets Mentioned

BETA
SRR2157163

Methods Mentioned

BETA
PCR
Assay
chips
PCA

Software Mentioned

Phylogenetic Reconstruction of ( PICRUSt )
PICRUSt script
py
R package Vegan
fastq
PICRUSt
Ion Torrent Server
Silva TestProbe
QIIME
glmnet

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.