Characterization of bacterial microbiota compositions along the intestinal tract in pigs and their interactions and functions

Scientific Reports
Daniel Crespo-PiazueloJosep M Folch

Abstract

In addition to its value in meat production, the pig is an interesting animal model for human digestive tract studies due to its physiological similarities. The aim of this study was to describe the microbiome composition, distribution and interaction along the Iberian pig intestinal tract and its role in whole-body energy homeostasis. The V3-V4 region of the 16S rRNA gene was amplified and sequenced from the microbiomes of five gut sections (duodenum, jejunum, ileum, and proximal and distal colon) in thirteen castrated male pigs. A total of 1,669 operational taxonomic units distributed in 179 genera were found among all samples. The two most abundant genera in the small intestine were Lactobacillus and Clostridium, while Prevotella was predominant in the colon. The colon samples were more similar among the pigs and richer in species than the small intestine samples were. In the small intestine, the metagenome prediction pointed to rapid internalization and conversion of the available simple carbohydrates for microbial proliferation and maintenance. In the colon, a competition among anaerobic bacteria for plant polysaccharide degradation to produce short chain fatty acids was found. This study confirms that the energy pathways ...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1997·The British Journal of Nutrition·H JørgensenB O Eggum
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·G HeJ L Zweier
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fredrik BäckhedJeffrey I Gordon
Mar 26, 2005·Science·Fredrik BäckhedJeffrey I Gordon
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew K BensonDaniel Pomp
Dec 20, 2011·Nature Reviews. Genetics·Justin KuczynskiRob Knight
Oct 3, 2012·PLoS Computational Biology·Jonathan Friedman, Eric J Alm
Dec 4, 2012·Nature Methods·Nicholas A BokulichJ Gregory Caporaso
Aug 27, 2013·Nature Biotechnology·Morgan G I LangilleCurtis Huttenhower
Oct 1, 2013·Nature Methods·Joseph N PaulsonMihai Pop
Apr 15, 2014·Nature Biotechnology·Harald Brüssow, Scott J Parkinson
Jan 7, 2015·Disease Models & Mechanisms·Thi Loan Anh NguyenJeroen Raes
Mar 3, 2015·Environmental Microbiology Reports·Núria MachPatricia Lepage
Mar 6, 2015·Frontiers in Microbiology·Brett Wagner MackenzieMichael W Taylor
May 21, 2015·ILAR Journal·Mei Wang, Sharon M Donovan
Aug 12, 2015·Nature Reviews. Endocrinology·Emanuel E CanforaEllen E Blaak
Sep 24, 2015·BMC Microbiology·Mackenzie Jonathan SlifierzJ Scott Weese
Oct 27, 2015·Nature Reviews. Microbiology·Gregory P DonaldsonSarkis K Mazmanian
Jan 1, 2016·Biochemical and Biophysical Research Communications·Ravi RanjanDavid L Perkins
Jun 10, 2016·Nature·Amir BashanYang-Yu Liu
Sep 20, 2016·Nature Microbiology·Liang XiaoJun Wang
May 21, 2017·Applied and Environmental Microbiology·Ursula M McCormackPeadar G Lawlor

❮ Previous
Next ❯

Citations

Oct 3, 2019·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Christian MalteccaFrancesco Tiezzi
Jul 16, 2019·Journal of Animal Science and Biotechnology·Nuria CanibeSam Abraham
Apr 17, 2019·International Journal of Molecular Sciences·Bishnu AdhikariYoung Min Kwon
Jul 19, 2019·Nutrients·Ronald D HillsCory R Theberge
Apr 17, 2020·Animals : an Open Access Journal From MDPI·Francesca Romana MassacciChiara Francesca Magistrali
Sep 30, 2020·Animals : an Open Access Journal From MDPI·Modestas RuzauskasValdas Jakstas
Jun 12, 2020·Scientific Reports·Özgün Candan Onarman UmuHenning Sørum
Mar 16, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Saad Al-MasaudiBernard Henrissat
Jul 31, 2020·Genetics, Selection, Evolution : GSE·Piush KhanalFrancesco Tiezzi
Mar 12, 2020·Frontiers in Microbiology·Vandana R PrabhuMuniyandi Nagarajan
Jul 3, 2020·Frontiers in Immunology·Jayne E WiardaCrystal L Loving
Oct 28, 2019·Frontiers in Microbiology·Ann M ArfkenKatie Lynn Summers
Mar 21, 2020·Frontiers in Microbiology·Sophie StolzenbachStig M Thamsborg
Sep 12, 2020·The British Journal of Nutrition·Carlos A MontoyaPaul J Moughan
Oct 18, 2020·Microorganisms·Samat AmatBenjamin P Willing
Dec 15, 2020·Current Pharmacology Reports·Joseph L Dempsey, Julia Yue Cui
Jan 21, 2021·Frontiers in Cell and Developmental Biology·Elisabeth UrbauerDirk Haller
Feb 7, 2021·Applied and Environmental Microbiology·Paiboon TunsagoolGunnaporn Suriyaphol
Aug 21, 2019·Physiology & Behavior·Narjis KraimiChristine Leterrier
Feb 21, 2021·Applied and Environmental Microbiology·Paul E MooteG Douglas Inglis
Apr 27, 2021·Journal of Dairy Science·Alejandro Saborío-MonteroOscar González-Recio
Jul 24, 2021·Applied Microbiology and Biotechnology·A CheccucciPaolo Trevisi
Aug 8, 2021·Animals : an Open Access Journal From MDPI·María Bernad-RocheRaúl Carlos Mainar-Jaime
Aug 11, 2021·Trends in Immunology·Dorothée L BertholdIrina A Udalova
Apr 17, 2020·Journal of Agricultural and Food Chemistry·Matthias KasimirHans-Ulrich Humpf

❮ Previous
Next ❯

Methods Mentioned

BETA
phosphotransferase
PCR
electrophoresis

Software Mentioned

VennDiagram R package
metagenomeSeq R package
DESeq2 R package
SPCIT
vegan
PICRUSt
Cytoscape
QIIME
NMDS
Sparse Correlations for Compositional data ( SparCC )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.