Characterization of bacteriocins from two Lactococcus lactis subsp. lactis isolates

Molecular Nutrition & Food Research
Oya AkçelikMustafa Akçelik

Abstract

In this study, bacteriocins from two Lactococcus lactis subsp. lactis isolates from raw milk samples in Turkey designated OC1 and OC2, respectively, were characterized and identified. The activity spectra of the bacteriocins were determined by using different indicator bacteria including Listeria, Bacillus and Staphylococcus spp. Bacteriocins were tested for their sensitivity to different enzymes, heat treatments and pH values. Loss of bacteriocin activities after alpha-amylase treatment suggested that they form aggregates with carbohydrates. Molecular masses of the purified bacteriocins were determined by SDS-PAGE. PCR amplification was carried out with specific primers for the detection of their structural genes. As a result of these studies, the two bacteriocins were characterized as nisin and lacticin 481, respectively. Examination of plasmid contents of the isolates and the results of plasmid curing and conjugation experiments showed that in L. lactis subsp. lactis OC1 strain the 39.7-kb plasmid is responsible for nisin production, lactose fermentation and proteolytic activity, whereas the 16.0-kb plasmid is responsible for lacticin 481 production and lactose fermentation in L. lactis subsp. lactis OC2 strain.

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Citations

Jan 13, 2009·Journal of Industrial Microbiology & Biotechnology·O SimşekMustafa Akçelik
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Archana KumariMustafa Akçelik
Nov 29, 2011·Journal of Microbiological Methods·Sunita J Macwana, Peter M Muriana
Nov 14, 2006·FEMS Microbiology Reviews·Alain DufourJean-Paul Le Pennec
Jul 21, 2009·Biotechnology Journal·Tunçer H Ozdamar
Nov 26, 2009·Journal of Applied Microbiology·J A Renye, G A Somkuti
Oct 23, 2013·Asian Pacific Journal of Tropical Medicine·D SenbagamB Senthilkumar
Aug 5, 2008·Letters in Applied Microbiology·F YanagidaY-S Chen
Sep 1, 2013·Probiotics and Antimicrobial Proteins·Asmat AhmadGires Usup

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