PMID: 2113083Jul 1, 1990Paper

Characterization of beta-amyloid precursor proteins with or without the protease-inhibitor domain using anti-peptide antibodies

Journal of Neurochemistry
E R SheltonH W Chan

Abstract

Alternative splicing of the transcript encoding the beta-amyloid precursor protein (BAPP) of Alzheimer's disease produces multiple mRNA species. Translation of these mRNAs predicts protein products of 770, 751, and 695 amino acids. The difference arises from the inclusion in BAPP-770/751 of a 56-residue insert region which is homologous to Kunitz-type protease inhibitors. We have prepared and affinity-purified anti-peptide antibodies that react specifically with either BAPP-770/751 (insert-specific) or BAPP-695 (junction-specific). A detectable level of the mRNA corresponding to the BAPP-770/751 protein was found in all cell lines tested. Immunoprecipitation of 35S-labeled proteins from these cell lines showed them to contain one or two Mr 105,000 bands reactive with the insert-specific serum, i-291. In contrast, only cos-7 cells and the human neuroblastoma cell line, IMR-32, contained mRNA species that encode the BAPP-695 protein, as shown by Northern analysis with a junction-spanning oligonucleotide probe. A band of Mr 95,000 was immunoprecipitated specifically from these two cell lines using the junction-specific serum, J-284. Indirect immunofluorescence labeling of cells corroborated these findings. All cells reacted with t...Continue Reading

References

Feb 11, 1988·Nature·R W Carrell
Nov 30, 1989·Biochemical and Biophysical Research Communications·M R PalmertS G Younkin
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D SchubertG Cole
May 15, 1989·Biochemical and Biophysical Research Communications·K TakioY Ihara
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M R PalmertS G Younkin
Feb 1, 1989·Biochemical Society Transactions·M Landon, M Kidd
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I Grundke-IqbalH M Wisniewski
Jan 25, 1989·Nucleic Acids Research·H G LemaireB Müller-Hill
Mar 5, 1987·The New England Journal of Medicine·S TsujiE I Ginns
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S B ZainC A Marotta
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N K RobakisH M Wisniewski
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D J SelkoeT Oltersdorf
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S W LeeA C Allison
Jan 11, 1988·Nucleic Acids Research·G H MurdochE E Manuelidis
Nov 1, 1987·Molecular and Cellular Biology·J PapkoffH E Varmus
May 16, 1984·Biochemical and Biophysical Research Communications·G G Glenner, C W Wong

❮ Previous
Next ❯

Citations

Jul 5, 1991·Journal of Immunological Methods·L M KhachigianC N Chesterman
Jan 1, 1992·Pharmacology & Therapeutics·A I BushC L Masters
Mar 1, 1991·Neurobiology of Aging·G M ColeT Saitoh
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J AdlerN N Dewji
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P CrasG Perry
Apr 1, 1994·The Journal of Comparative Neurology·J G BeesonF H Gage

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.