Characterization of beta-keratins and associated proteins in adult and regenerating epidermis of lizards

Tissue & Cell
L AlibardiM Toni

Abstract

Reptilian epidermis contains two types of keratin, soft (alpha) and hard (beta). The biosynthesis and molecular weight of beta-keratin during differentiation of lizard epidermis have been studied by autoradiography, immunocytochemistry and immunoblotting. Tritiated proline is mainly incorporated into differentiating and maturing beta-keratin cells with a pattern similar to that observed after immunostaining with a chicken beta-keratin antibody. While the antibody labels a mature form of beta-keratin incorporated in large filaments, the autoradiographic analysis shows that beta-keratin is produced within the first 30 min in ribosomes, and is later packed into large filaments. Also the dermis incorporates high amount of proline for the synthesis of collagen. The skin was separated into epidermis and dermis, which were analyzed separately by protein extraction and electrophoresis. In the epidermal extract proline-labeled proteic bands at 10, 15, 18-20, 42-45, 52-56, 85-90 and 120 kDa appear at 1, 3 and 5 h post-injection. The comparison with the dermal extract shows only the 85-90 and 120 kDa bands, which correspond to collagen. Probably the glycine-rich sequences of collagen present also in beta-keratins are weakly recognized by ...Continue Reading

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Citations

Dec 16, 2006·Zoology : Analysis of Complex Systems, ZACS·Mattia Toni, Lorenzo Alibardi
Oct 12, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Luisa Dalla ValleLorenzo Alibardi
Sep 8, 2011·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Matthew J Greenwold, Roger H Sawyer
Apr 8, 2006·Zoology : Analysis of Complex Systems, ZACS·Lorenzo Alibardi, Mattia Toni
Apr 6, 2006·Progress in Histochemistry and Cytochemistry·Lorenzo Alibardi, Mattia Toni
Apr 15, 2006·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Lorenzo Alibardi, Mattia Toni
Jun 9, 2006·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Lorenzo AlibardiMattia Toni
Oct 17, 2006·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Lorenzo Alibardi, Mattia Toni
Aug 12, 2005·Journal of Neurophysiology·Christian J SumnerSusan E Shore
Oct 26, 2007·Journal of Neurophysiology·Veeramuthu Balakrishnan, Laurence O Trussell

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