Characterization of bonded stationary phase performance as a function of qualitative and quantitative chromatographic factors in chaotropic chromatography with risperidone and its impurities as model substances

Analytical and Bioanalytical Chemistry
Jelena ČolovićAnđelija Malenović

Abstract

Numerous stationary phases have been developed with the aim to provide desired performances during chromatographic analysis of the basic solutes in their protonated form. In this work, the procedure for the characterization of bonded stationary phase performance, when both qualitative and quantitative chromatographic factors were varied in chaotropic chromatography, was proposed. Risperidone and its three impurities were selected as model substances, while acetonitrile content in the mobile phase (20-30%), the pH of the aqueous phase (3.00-5.00), the content of chaotropic agents in the aqueous phase (10-100 mM), type of chaotropic agent (NaClO4, CF3COONa), and stationary phase type (Zorbax Eclipse XDB, Zorbax Extend) were studied as chromatographic factors. The proposed procedure implies the combination of D-optimal experimental design, indirect modeling, and polynomial-modified Gaussian model, while grid point search method was selected for the final choice of the experimental conditions which lead to the best possible stationary phase performance for basic solutes. Good agreement between experimentally obtained chromatogram and simulated chromatogram for chosen experimental conditions (25% acetonitrile, 75 mM of NaClO4, pH 4....Continue Reading

References

Nov 7, 2001·Journal of Chromatography. a·V B Di Marco, G G Bombi
Feb 3, 2004·Journal of Chromatography. a·J J Baeza-Baeza, M C García-Alvarez-Coque
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Jun 3, 2011·Journal of Pharmaceutical and Biomedical Analysis·Bieke Dejaegher, Yvan Vander Heyden
Jun 23, 2012·Analytical and Bioanalytical Chemistry·Szymon BocianBoguslaw Buszewski
Mar 5, 2013·Journal of Chromatography. a·J J Baeza-BaezaM C García-Álvarez-Coque

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