Characterization of Borrelia burgdorferi associated antigens by monoclonal antibodies

Immunobiology
M D KramerM M Simon

Abstract

In this paper, we present a series of murine mAb recognizing B. burgdorferi antigens. The antibodies were characterized by immuno-blotting and immuno-fluorescence studies using isolates of B. burgdorferi from North America and Europe, respectively. Moreover, reactivity of the antibodies with recombinant B. burgdorferi flagellin and OspA was studied. The results suggest these anti-B. burgdorferi mAb as valuable tools for the serological analysis of B. burgdorferi isolates and for affinity-purification of the respective proteins. Moreover, these mAb appear suitable to classify antigenic variants of B. burgdorferi and to study the protective capacity of antibodies in a murine model for B. burgdorferi infection.

References

May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·U E SchaibleM M Simon
Nov 1, 1985·Neurology·A R PachnerC J Johnson
Jan 1, 1988·Annals of the New York Academy of Sciences·B WilskeM Kramer
May 11, 1989·Nucleic Acids Research·G S GassmannR Wallich
Oct 1, 1984·The American Journal of Medicine·C M MoffatJ M Dwyer

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Citations

Apr 28, 2004·Medical Microbiology and Immunology·Sharon LedererMarkus M Simon
May 1, 1996·FEMS Microbiology Letters·C Grewe, J H Nuske
Jun 1, 1993·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·M Moskophidis, B Luther
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·H FuchsM D Kramer
Sep 1, 1992·The British Journal of Dermatology·H M GisslerM D Kramer
Jun 1, 1993·The British Journal of Dermatology·H M GisslerM D Kramer
Apr 19, 2013·Clinical and Vaccine Immunology : CVI·Angela M FlodenCatherine A Brissette
May 23, 2003·Infection and Immunity·Timothy R CrotherMichael A Lovett
Aug 24, 2004·Infection and Immunity·Timothy R CrotherMichael A Lovett
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·W ZhongM M Simon
Jan 30, 2015·FEBS Open Bio·Philipp KolbMichael Nassal
Mar 1, 1994·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·S Leuba-GarciaL Gern
Mar 6, 2015·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Leandro LemgruberFriedrich Frischknecht
Feb 1, 1996·The British Journal of Dermatology·P BreierE Aberer
Jul 19, 2013·The Journal of Biological Chemistry·Arno KoenigsPeter Kraiczy
Feb 28, 2002·The Journal of Infectious Diseases·Benjamin J LuftCatherine L Lawson
Oct 1, 1992·Infection and Immunity·M G RittigG R Burmester
Feb 1, 1994·Journal of Clinical Microbiology·T J SullivanS L Balaban
Jan 1, 1995·Journal of Clinical Microbiology·L M NohlmansC P van Boven

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